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Team:ETH Zurich/expresults/qs/tab-plux
From 2014.igem.org
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|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062]) | |+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062]) | ||
| - | |+ | + | |+ In all the measurements conducted to create this matrix the promoter pLux was the basis and was induced in six different variations shown. |
| + | The dark blue points in the graph top left show the activation of gene expression when pLux is induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. The observed transition occurs at a concentration of approximately 1 nM 3OC6-HSL. The light-blue curve plotted shows modeling data of pLux induced by 3OC6-HSL (Lux-AHL) binding to the corresponding LuxR regulator. This curve from the model and the dark blue data point gained in experiments were plotted as a reference in all the other graphs describing pLux. | ||
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| + | Crosstalk can be observed for the cases where the 3OC12-HSL (Las-AHL) binds the LuxR regulator. Additionally for 3OC12-HSL binding to its corresponding regulator LasR and then binding to the pLux as seen in the middle of the top row and center of the matrix. | ||
| + | For the case of Las-AHL binding the LasR and subsequently the promoter pLux, the transition occurs at 1 nM and reaches 0.5 fold the fluorescence as pLux induced by 3OC6-HSL binding LuxR. | ||
| + | In the case of 3OC12-HSL binding LuxR and inducing the promoter pLux, the transition is observed at approximately 100 nM and severe crosstalk is observed. | ||
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| + | The promoter pLux was not tested in combination with the RhlR regulator and the C4-HSL. | ||
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| + | Observation of C4-HSL has shown, that there is no significant crosstalk with the LuxR and LasR regulators binding C4-HSL and subsequently to pLux. This is indicated on top right and middle right graphs. | ||
|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]] | |style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerLux.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]] | ||
|[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]] | |[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]] | ||
Revision as of 18:37, 17 October 2014
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