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Team:UFAM Brazil/9-29-2014
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Revision as of 18:22, 17 October 2014
09/29/2014 | ||
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Today we returned to the laboratory of Mineral Analyzes on CPRM to make our calibration curve that will work as a pattern for mercury quantification on experiments with constructions of the binding protein to mercury (MBP): | ||
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Besides that, we also decided to digest each bio brick to be submitted for the competition in a more rigorous way. Starting with BB_Bioremediation, with the following system: | ||
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Electrophoretic digestion profile of BB_Bioremediation: | ||
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1 : BB_Bioremediation in PSB1C3 not digested 2: BB_Bioremediation in PSB1C3 digested with EcoRI 3: BB_Bioremediation in PSB1C3 digested with PstI 4: BB_Bioremediation in PSB1C3 digested with EcoRI and PstI 5 : BB_Bioremediation in PSB1C3 digested with Xba 6: BB_Bioremediation in PSBK from JM110 digested with Xba 7: BB_Bioremediation in PSBK from JM110 digested with Spe 8: BB_Bioremediation in PSBK from JM110 digested with Xba + Spe. |
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