Team:TCU Taiwan/Notebook

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Notebook

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iGEM materials

Bacteria medium LB(Luria-Bertani) broth: 1% Trytone, 1% NaCl, 0.5% Yeast extract in the ddH2O LB agar plate:LB broth add 1.5% agar.If you want to add selection marker example for ampicillin or chloramphenicol. To culture *E.coli*, ampicillin is added 100 mg/ml for usage, chloramphenicol is added 30 mg/ml for usage, kanamycin is added 70 mg/ml for usage

Plasmid isolation Solution I: 50 mM glucose、25 mM Tris-HCl (pH 8.0)、10 mM EDTA Solution II:0.2N NaOH and 1% SDS(Sodium dodecyl sulfate ) mix Solution III: 5 M potassium acetate (pH4.8), 11.5% glacial acetic acid RNase A(10 mg/ml) Phenol chloroform Chloroform 95% Ethanol Tris-EDTA(TE)buffer:10 mM Tris-Cl(pH 7.5) 1 mM EDTA pBluescript SK(-) BBa_K1218011 BBa_K914003 BBa_I13521

Quick screening Quick screening buffer(Tris-Cl pH 12.6, 3% SDS) Phenol chloroform *Chloroform

Enzyme digestion T4 ligase 10X ligation buffer *EcoRI *PstI *SpeI *XbaI *BSA 0.1%

Bacteria strains and others DH5a JM101 0.8% and 2% agarose gel 95% Ethanol 6X loading dye 1.5 ml centrifuge tube 250 ml flask M13KO7(11011 pfu/ml)from NEW ENGLAND BioLabs*

Polymerase chain reaction *Top DNA polymerase dNTP(dATP, dCTP, dGTP, dTTP) 250 mM Tris-HCl(pH9.0) 10 mM KCl 30 mM MgCl2 1.5 mM

Instruments VCM-620, laminal flow Biofuge pico, Heraeus * OS1500-H, TSK Oribital Shaking Iucubator * Biowave * Vortex-GENE 2 * Autoclave AS-1060L * GenAmp PCR System 2400, Applied Biosystems BU-420, YIHDER 5804R, eppendorf Centrifuge


Life Records

Study Together

Conference in NCTU

Trip in Chihsingtan Beach

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Team Members	Project	Parts	Human Pratics	Modeling	Safety	Notebook	Attributions

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