Team:Peking/Attributions

Team Members

Binding: Zhang Zijian&Meng Liuyi

An important improvement was constructing by these two members. They tested binding efficiency between E.coli and cyanobacteria. Equipped with fluorescence microscope, they calculated binding efficiency of protein-algae and E.coli-algae interaction.

Killing: Yao Lanqiu&Lu Xiaoyu

Killing cyanobacteria is the core of our project, so YAO and LU spent a lot of time searching for a weapon. Finally, lysozyme was chosen from tens of candidates. They measured cyanobacteria growth curves under different conditions. Killing effects on different species of cyanobacteria were tested by them, which showed the wide applicability of our project.

Degradation: Jin Yuji&Zhang Fengyu

Algae toxins are serious threats from algal bloom. Degradation group found a enzyme MlrA that can degrade it. A sophisticated experiment assay was established to test degradation efficiency. Several signals were tried for the sake of secreting MlrA outside cells.

Suicide: Wu Jie

Considering biosafety, a suicide system was introduced into our E.coli. Wu Jie made effort transferring induction suicide system into E.coli. He tested growth curves of E.coli under variant inducing conditions.

Gas vesicle: Sherry Teng

Sherry came to Peking iGEM during the summer and was taking the gas vesicle experiments. She used strains from OUC-China and did a lot to make them float. More than experiments, Sherry polished Peking‘s wiki drafts.

Protein purification: Mu Xuelang&Zhu Shiyu

To get quantitative data of binding and degradation, we planned to use purified proteins to do experiments. These two members spent the whole summer working with proteins. The purified proteins supported subsequent experiments and provided reliable data.

Modelling: Wang Chenggong&Ni Congjian

Using mathematic methods modelling biology phenomenon is what we used this year. We established 3 models, binding model, application model, and cellular burden, corresponding binding experiments，killing experiments, and cell burden estimation respectively. Besides model, Wang as team leader, contributed much confirming our experimetns progress. And he build the framework of our wiki.

Art design: Huang Xiaoyu

Huang Xiaoyu designed logo, icons for each group, and wiki for Peking iGEM 2014. What’s more, ppt template, poster and banner were from her hands. And she beautify our photos.

Team leader: Yu Congyu

As team leader of Peking iGEM 2014, YU and vice team leader organized experiments and set timeline for each group. He finished the human practice part of our project and led a field investigation to Taihu lake to get firsthand material of algal bloom.

Acknowledgements

Besides team members, we wanna thanks these people below for their contribution to our project:

Instructors

Ouyang Qi

Kang Li

Liu Shiyu

Advisor

Zheng Pu

High school student

Du Haoxing

Undergraduate

Hu Jiashuai

Graduates

Cheng Shuobing

Zhang Haoqian

Researchers

NIGLAS

Prof. Kong Fanxiang

Dr. Shen Ji

Dr. Chen Yafen

Dr.Shen Xiaoli

Dr. Xie Liqiang

Dr. Zhang Ming

Dr. Yang Hongwei

Station Master Assistant: Shen Ruijie

These people above provided Peking iGEM 2014 constructive suggestion on Microcysin degradation and cyanobacteria proliferation. And we got the Microcystis aeruginosa strain PCC 7806 and water samples from them.

Peking University

Prof. Chen Jianguo

Prof. Ouyang Qi

Prof. Lai luhua

Prof. Xu Chongren

Associate Prof. He Xinqiang

Dr. Wei Ping

Dr. Li Qing

Dr. Luo Chunxiong

Institute of Microbiology, Chinese Academy of Sciences

Dr. Lou Chunbo

These teachers offered us many experiments equipment . Without their help, we shall never finish so many experiments to make our ideas into reality. During experiments, these teachers also gave us much constructive advice, which assisted us a lot.