Revision as of 13:55, 15 July 2014

Thursday 3rd July

by Mathias

In 2ml Eppendorf :

4 eppendorf MG1655 (0.1ml + 50µl CaCl)

4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)

Add 100µl of the different phages. Wait 20 minutes!

Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M Wait 1 or 2 hours at 37°C with agitation

Pour in LB Kan petri dishes, incubate at 37°C. (Turn the petri dishes over when there is no more liquid in!)

by Fabio, Maher, Mathieu, Marie and Romain

People there:

Instructors : Alice & Solenne. Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.

@@ Line 4: / Line 4: @@
 ===1 - Phages P1 transduction in MG1655 and MG1655Z1===
+''by Mathias''
 In 2ml Eppendorf :
@@ Line 15: / Line 17: @@
 '''Wait 1 or 2 hours at 37°C with agitation'''
-Pour in LB Kan petri dishes, incubate at 37°C.(''Turn the petri dishes over when there is no more liquid in!'')
+Pour in LB Kan petri dishes, incubate at 37°C. (''Turn the petri dishes over when there is no more liquid in!'')
+===2 - Plasmid DNA Purification===
+''by Fabio, Maher, Mathieu, Marie and Romain''
+* K773002 (x2)
+* K517003 (x2)
+* K773002 (x2)
+* K592011 (x2)
+* K1033910 (x2)
+* K592009 (x2)
+* K786002 (x2)
+* K786003 (x2)
+* J45014 (x2)
+* I9026 (x2)
+[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol]