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Team:Evry/Notebook/Transposons/09.02.14
From 2014.igem.org
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PCR Tn10 with primer biobrick 64 and 65 is accomplished. After migration a bands at 1200 bp. This product is purified and send to sequence. | PCR Tn10 with primer biobrick 64 and 65 is accomplished. After migration a bands at 1200 bp. This product is purified and send to sequence. | ||
Revision as of 17:48, 17 October 2014
Amplification of pNK2 muted
The DH5α is transformed with this new product dialysed called pNK2 (2).Transformation Pseudovibrio with pNK2 and sowed in selection medium kanamycin (50µg/mL) for to have a fresh transformed cells.
