Team:Evry/Notebook/Transposons/09.02.14

From 2014.igem.org

(Difference between revisions)
SophiaB (Talk | contribs)
(Created page with "<html> <div class="cd-timeline-block"> </html> {{:Team:Evry/Notebook/CellCharacterization/Transformation/TopImage}} <html> <div class="cd-timeline-content"> <p align="just...")
Newer edit →

Revision as of 17:48, 17 October 2014

Picture

Amplification of pNK2 muted

The DH5α is transformed with this new product dialysed called pNK2 (2).
Transformation Pseudovibrio with pNK2 and sowed in selection medium kanamycin (50µg/mL) for to have a fresh transformed cells.

transposas Tn10

PCR Tn10 with primer biobrick 64 and 65 is accomplished. After migration a bands at 1200 bp. This product is purified and send to sequence.

sep 02