Team:NUDT CHINA/Safety

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<p><i>Parts</i></p>
 
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<a href="https://2014.igem.org/wiki/index.php?title=Team:NUDT_CHINA/Safety&action=edit"> Click here to edit this page!</a>
 
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<h1 >WELCOME TO iGEM 2014! </h1>
 
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<p>Your team has been approved and you are ready to start the iGEM season!
 
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:NUDT_CHINA/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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<a href="https://2014.igem.org/Team:NUDT_CHINA/Safety"style=" color:#000000"> Safety </a></td>
 
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<a href="https://2014.igem.org/Team:NUDT_CHINA/Attributions"style="color:#000000"> Attributions </a></td>
 
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
 
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<p><i>Parts</i></p>
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<p>Our parts are all assembled with materials from the distribution or synthesized based on known sequence.
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For more detaile, please read the ’’part’’ section of our wiki.</p>
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<p><h5>Institutional Rules and Regulations</h5></p>
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<p>The NUDT's Commission for Construction of Biological Sciences is responsible for bio-security in our institution. We have discussed the project with the teachers of this commission, and gotten their approval. Three extra teachers led by the deputy chief of DCB(the Department of Chemistry and Biology) were designated to provide us assistance if necessary and monitor us on our implementation on the lab bio-safety protocols at the same time.</p>
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<p><h5>Training</h5></p>
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<h3> Edit this page!</h3>
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<p>All team members were required to attend safety training focused on: </p>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
 
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<li> <a href=" ">Learn about lab Safety for Today</a></li>  
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<li>General biosafety principles.</li>
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<li> <a href="">Learn about Safety for the future of your project.</a></li>  
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<li>The proper use of containment equipment (Biological safety (laminar flow) cabinets and delimited working space when required) as well as personal protective equipment (PPE) (White coat and gloves).</li>
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<li>Risk assessment of experiments involving: Recombinant DNA, ethidium bromide, other bio hazardous materials.</li>
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<li>Proper Biological waste disposal.</li>
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<h3> Your Lab </h3>
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<p><h5>Lab security</h5></p>
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<p><i>toxic chemical reagents EB</i>
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<p>Ethidium Bromide (EB) is a very toxic agent. However, because it’s high efficiency and low cost, our lab still use this molecule as nucleic acid staining molecule. To prevent it from hurting us, our lab requires everyone in the electrophoresis Rome to wear a second pair of gloves. Also, Wasted gel were collected and properly disposed.</p>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
 
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Image:Example2_Lab_1.png|The building our lab is in!
 
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
 
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
 
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
 
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
 
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
 
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
 
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
 
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
 
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
 
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
 
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<a href="https://2014.igem.org/wiki/index.php?title=Team:NUDT_CHINA/Safety&action=edit"> Click here to edit this page!</a>
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<a href="https://2014.igem.org/Special:Upload">Click here to upload! </a>
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Revision as of 17:36, 17 October 2014


Safety

Organisms and Parts

Organisms

This year, we used different species of E.coli in our project. Different Genotype provided us different choices when planning our experiment. Based on our project, following 4 species of E.coli was used in our project. All of these species are common and widely used in the field of molecular clone.

StrainSpecies nameGenotypeRish GroupRish Group SourceDisease risk to humans
DH5αEscherichia coliF-,φ80dlacZΔM15,Δ(lacZYA-argF)U169,deoR,recA1,endA1,hsdR17(rk-,mk+),phoA,supE44,λ-,thi-1,gyrA96,relA11PSDSlow infectious dose
Top10Escherichia coliF-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16, rpsL(StrR), endA1, λ-1PSDSlow infectious dose
BL21(DE3)Escherichia coliF-,ompT, hsdS(rBB-mB-),gal, dcm(DE3)1PSDSlow infectious dose
MC1061/P3Escherichia coliF-, hsdR, (rk-, mk+) araD139, Δ(araABC-leu)7679, galU, galK, ΔlacX74, rpsL, thi, mcrB, {P3: KanR, AmpR,(am) TetR, (am)}1PSDSlow infectious dose

Parts

Our parts are all assembled with materials from the distribution or synthesized based on known sequence. For more detaile, please read the ’’part’’ section of our wiki.

Institutional Rules and Regulations

The NUDT's Commission for Construction of Biological Sciences is responsible for bio-security in our institution. We have discussed the project with the teachers of this commission, and gotten their approval. Three extra teachers led by the deputy chief of DCB(the Department of Chemistry and Biology) were designated to provide us assistance if necessary and monitor us on our implementation on the lab bio-safety protocols at the same time.

Training

All team members were required to attend safety training focused on:

  • General biosafety principles.
  • The proper use of containment equipment (Biological safety (laminar flow) cabinets and delimited working space when required) as well as personal protective equipment (PPE) (White coat and gloves).
  • Risk assessment of experiments involving: Recombinant DNA, ethidium bromide, other bio hazardous materials.
  • Proper Biological waste disposal.

Lab security

toxic chemical reagents EB

Ethidium Bromide (EB) is a very toxic agent. However, because it’s high efficiency and low cost, our lab still use this molecule as nucleic acid staining molecule. To prevent it from hurting us, our lab requires everyone in the electrophoresis Rome to wear a second pair of gloves. Also, Wasted gel were collected and properly disposed.

Click here to edit this page! Click here to upload!