Team:UANL Mty-Mexico/wetlab/mini project

From 2014.igem.org

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Revision as of 17:24, 17 October 2014

Miniproject

Introduction

It is know that TetR (like others transcriptional repressors) can allow a basal expression. Because of that, it would be a good idea to reduce that basal expression using a molecular tool which does not cause noise to circuits and systems.

The last year our team participated with the project “Thermo coli” (Team: UANL_Mty-Mexico). The main biobrick (K1140006) that we used was composed for the fluorescent protein mCherry (E1010) regulated by the ptet promoter (R0040) and a 37°C thermometer. This year we decided to use that biobrick part to develop a mini-project. We want to proveif it is possible to enhance the repression of a gene by a combined use of the repressor protein TetR (transcriptional regulation) and a RNA thermometer (post-transcriptional regulation).

Constructions

The fluorescent part was synthetized based on the promoter ptet (R0040), a RBS fused to a RNA thermometer which was obtained on a previous work (Neupertet al, 2008), the mCherry (E1010) and the transcriptional terminator T7 (B0010-B0012).

Figure 1. Here is shown the structure of the part (K1140006).

The game was made with the program “Game Maker” which allows you, the programmer, to make its own game without the necessity of a specific programming language.

GALLERY

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-Software</div>
+Miniproject</div>
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-<table id="subtitle" width="100%">
-<tr>
-<td>
-<div class="Estilo6">
-Game
-</div>
-</td>
-</tr>
-</table>
 <table style="background-color: #FFFFFF;" width="100%" id="texto">
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-<td style="padding:12px"><p align="justify">iGEM CIDEB UANL 2013 team, in order to make our pet “Vip-thor” known, to other iGEM teams, we made a game in which the protagonist is out pet. This game, similar to PACMAN in which Vip-thor must go through 5 levels, in all of them he aims to collect  the lost pesticide that was given to his grandfather, form the iGEM CIDEB UANL 2013, for his lands. </p>
+<td style="padding:12px"><p align="justify">
-<p align="justify">The game is about catching Vip-thor (the player) in different levels of difficulty and was programmed in a way that the ghost or ghosts don’t have a predetermined cycle, and the same level could have different outcomes, this, to help level 1 not being easier than two and to help it  to be diverse according to each match.</p>
+<p align="center"><b>Introduction</b></p><br>
+<p>It is know that TetR (like others transcriptional repressors) can allow a basal expression. Because of that, it would be a good idea to reduce that basal expression using a molecular tool which does not cause noise to circuits and systems.</p>
+<p>The last year our team participated with the project “Thermo coli” (<a href="https://2013.igem.org/Team:UANL_Mty-Mexico">Team: UANL_Mty-Mexico</a>). The main biobrick (K1140006) that we used was composed for the fluorescent protein mCherry (E1010) regulated by the ptet promoter (R0040) and a 37°C thermometer. This year we decided to use that biobrick part to develop a mini-project. We want to proveif it is possible to enhance the repression of a gene by a combined use of the repressor protein TetR (transcriptional regulation) and a RNA thermometer (post-transcriptional regulation).</p>
+<p align="center"><b>Constructions</b></p><br>
+<p>The fluorescent part was synthetized based on the promoter ptet (R0040), a RBS fused to a RNA thermometer which was obtained on a previous work (Neupertet al, 2008), the mCherry (E1010) and the transcriptional terminator T7 (B0010-B0012). </p>
+<figure> <center>
+  <img src="https://static.igem.org/mediawiki/2014/c/ce/Miniprojectuanl_1.jpg" width=400px>
+  <figcaption><span class="text-muted"><font size="2">Figure 1. Here is shown the structure of the part (K1140006).
+ </span></font> <br></figcaption>
+</figure> </center>
+</p>
 </td>
 <td style="padding:12px">