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Team:USyd-Australia/Project/Design
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| - | <img src="https://static.igem.org/mediawiki/2014/c/c4/USyd-Australia_Integron_Design-Produce_Cassettes.png" width="60%" align="center"><br> <p>Gene Cassettes are the carriers of genetic information in our design. They are small, modular chunks of DNA containing a gene flanked by AttC sites. Cassettes, unlike plasmids, are non-replicative and so do not require extra genes to modulate or initiate replication. To create a cassette simply requires the use of primers to create half AttC sites at either end and circularizing the product through <a href="https://2014.igem.org/Team:USyd-Australia/Project/Protocols#gibson">Gibson Assembly</a> or in other ways.</p> | + | <img src="https://static.igem.org/mediawiki/2014/c/c4/USyd-Australia_Integron_Design-Produce_Cassettes.png" width="60%" align="center"><br> <p>Gene Cassettes are the carriers of genetic information in our design. They are small, modular chunks of DNA containing a gene flanked by AttC sites. Cassettes, unlike plasmids, are non-replicative and so do not require extra genes to modulate or initiate replication. To create a cassette simply requires the use of primers to create half AttC sites at either end and circularizing the product through <a href="https://2014.igem.org/Team:USyd-Australia/Project/Protocols#gibson">Gibson Assembly</a> or in other ways.</p><br><h2> </h2> |
<img src="https://static.igem.org/mediawiki/2014/1/1f/USyd-Australia_Integron_Design-Express_Integrase.png" width="60%"> | <img src="https://static.igem.org/mediawiki/2014/1/1f/USyd-Australia_Integron_Design-Express_Integrase.png" width="60%"> | ||
<img src="https://static.igem.org/mediawiki/2014/5/55/USyd-Australia_Integron_design-add_cassettes.png" width="60%"> | <img src="https://static.igem.org/mediawiki/2014/5/55/USyd-Australia_Integron_design-add_cassettes.png" width="60%"> | ||
Revision as of 17:12, 17 October 2014
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Project Design
Gene Cassettes are the carriers of genetic information in our design. They are small, modular chunks of DNA containing a gene flanked by AttC sites. Cassettes, unlike plasmids, are non-replicative and so do not require extra genes to modulate or initiate replication. To create a cassette simply requires the use of primers to create half AttC sites at either end and circularizing the product through Gibson Assembly or in other ways.
With thanks to:
