Team:USyd-Australia/Project/Design

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<td><tr> <img src="https://static.igem.org/mediawiki/2014/c/c4/USyd-Australia_Integron_Design-Produce_Cassettes.png"><br> Gene Cassettes are the carriers of genetic information in our design. They are small, modular chunks of DNA containing a gene flanked by AttC sites. Cassettes, unlike plasmids, are non-replicative and so do not require extra genes to modulate or initiate replication. To create a cassette simply requires the use of primers to create half AttC sites at either end and circularizing the product through <a href="https://2014.igem.org/Team:USyd-Australia/Project/Protocols#gibson">Gibson Assembly</a> or in other ways.</tr>
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<td><tr> <img src="https://static.igem.org/mediawiki/2014/c/c4/USyd-Australia_Integron_Design-Produce_Cassettes.png" width="80%" align="center"><br> Gene Cassettes are the carriers of genetic information in our design. They are small, modular chunks of DNA containing a gene flanked by AttC sites. Cassettes, unlike plasmids, are non-replicative and so do not require extra genes to modulate or initiate replication. To create a cassette simply requires the use of primers to create half AttC sites at either end and circularizing the product through <a href="https://2014.igem.org/Team:USyd-Australia/Project/Protocols#gibson">Gibson Assembly</a> or in other ways.</tr>
<img src="https://static.igem.org/mediawiki/2014/1/1f/USyd-Australia_Integron_Design-Express_Integrase.png">
<img src="https://static.igem.org/mediawiki/2014/1/1f/USyd-Australia_Integron_Design-Express_Integrase.png">
<img src="https://static.igem.org/mediawiki/2014/5/55/USyd-Australia_Integron_design-add_cassettes.png">
<img src="https://static.igem.org/mediawiki/2014/5/55/USyd-Australia_Integron_design-add_cassettes.png">

Revision as of 16:57, 17 October 2014

iGEM_Link



Gene Cassettes are the carriers of genetic information in our design. They are small, modular chunks of DNA containing a gene flanked by AttC sites. Cassettes, unlike plasmids, are non-replicative and so do not require extra genes to modulate or initiate replication. To create a cassette simply requires the use of primers to create half AttC sites at either end and circularizing the product through Gibson Assembly or in other ways.

Project Design

With thanks to:

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