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Team:Groningen/Template/MODULE/projects/toolbox/promoters
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For expressing genes in <i>L. lactis</i>, promoters are needed. | For expressing genes in <i>L. lactis</i>, promoters are needed. | ||
Together with the <a href="http://parts.igem.org/Part:BBa_B0034">most standard RBS</a> and the <a href="http://parts.igem.org/Part:BBa_B0015">standard double terminator</a> they get genes transcribed and translated. The set of promoters that is used for the <i>L. lactis</i> toolbox is the | Together with the <a href="http://parts.igem.org/Part:BBa_B0034">most standard RBS</a> and the <a href="http://parts.igem.org/Part:BBa_B0015">standard double terminator</a> they get genes transcribed and translated. The set of promoters that is used for the <i>L. lactis</i> toolbox is the | ||
| - | promoter collection that was BioBricked by the Uppsala iGEM team in 2013. | + | promoter collection that was BioBricked by the Uppsala iGEM team in 2013. The set |
| + | consists of the promoters <a href="http://parts.igem.org/Part:BBa_K1033219"> CP1</a>, <a href="http://parts.igem.org/Part:BBa_K1033220">CP8</a>, <a href="http://parts.igem.org/Part:BBa_K1033221">CP11</a>, <a href="http://parts.igem.org/Part:BBa_K1033222">CP29</a>, | ||
| + | <a href="http://parts.igem.org/Part:BBa_K1033223">CP30</a>, <a href="http://parts.igem.org/Part:BBa_K1033224">CP41</a> and <a href="http://parts.igem.org/Part:BBa_K1033225">CP44</a>. | ||
The promoters originate from <i>L. lactis</i> and have first been described | The promoters originate from <i>L. lactis</i> and have first been described | ||
by Jensen and Hammer in 1998.<sup>1</sup> | by Jensen and Hammer in 1998.<sup>1</sup> | ||
Latest revision as of 16:56, 17 October 2014
Promoters
For expressing genes in L. lactis, promoters are needed.
Together with the most standard RBS and the standard double terminator they get genes transcribed and translated. The set of promoters that is used for the L. lactis toolbox is the
promoter collection that was BioBricked by the Uppsala iGEM team in 2013. The set
consists of the promoters CP1, CP8, CP11, CP29,
CP30, CP41 and CP44.
The promoters originate from L. lactis and have first been described
by Jensen and Hammer in 1998.1
The promoters were placed in front of a fluorescent marker in L. lactis,
so their relative strength is known.
