Template:Nevada/TheAuxinSystem

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The BAITswitch


The Bioorthoginal Auxin Induceable Trigger Switch


            

        








        
        
        

            
            
                
                


                    
                    


                        
                        


                            
                            

                            



                            
                            


                                
                                
                                    
                                



                                
                                

                                    
                                    
                                        
                                            
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                                    Categories
                                


                                
                                
                                    
                                        
                                            
                                                Begining of Research
                                            
                                        
                                        
                                            
                                                The Auxin System
                                            
                                        
                                        
                                            
                                                The Coronatine System
                                            
                                        
                                        
                                            
                                                Results & Future Plans
                                            
                                        
                                        
                                    
                                


                            



                            
                            

                                
                                
                                    Nevada iGEM Parts
                                


                                
                                

                                    
                                    


                                                                    
                            

                                
                                

                                    
                                    
                                        Jaz1 is a degron tag utilized in the coronatine pathway. The protein of interest is tagged with Jaz1; when coronatine is added to the system, the protein and its tag are linked to the E3 ubiquitin ligase, COI1. Click the picture or the name below to be taken to the parts registry page.
                                    


                                    
                                    
                                        Jaz1 	
                                    


                                


                                                                
                                
                                    
                                


                                        



                                        
                                        

                                            
                                            

                                                
                                                
                                                    RFP-Jaz1 is a degron tag utilized in the coronatine pathway. The protein of interest is tagged with RFP-Jaz1; when coronatine is added to the system, the protein and its tag are linked to the E3 ubiquitin ligase, COI1. Click the picture or the name below to be taken to the parts registry page.
                                                


                                                
                                                
                                                     RFP-Jaz1
                                                


                                            


                                            
                                            
                                                
                                            


                                        



                                        
                                        

                                            
                                            

                                                
                                                
                                                    Jaz6 is a degron tag utilized in the coronatine pathway. The protein of interest is tagged with Jaz6; when coronatine is added to the system, the protein and its tag are linked to the E3 ubiquitin ligase, COI1. Click the picture or the name below to be taken to the parts registry page.
                                                


                                                
                                                
                                                                                            Jaz6
                                                


                                            


                                            
                                            
                                                
                                            


                                        



                                        
                                        

                                            
                                            

                                                
                                                
                                                    COI-1 or Coronatine Insensitive-1 recruits JAZ-1 or JAZ-6 tagged substrates in the presence of Coronatine for degradation by the Proteasome.
                                                


                                                
                                                
COI1
                                                


                                            


                                            
                                            
                                                 
                                            


                                        

                            

                                
                                

                                    
                                    
                                        TiRI is an ubuquitinase E3 that recognizes AID-tagged proteins and trigger their ubiquitination for further degradation. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.
This part takes place in a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use . However the patent does not cover the use for oviparian such as frogs and chicken.
                                    


                                    
                                    
                                        TIR-1 	
                                    


                                

                                
                                
                                    
                                


                            

                           

                                
                                

                                    
                                    
                                        This part is a GFP coding sequence fused to an AID tag for its recognition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. This is designed to be used in pSB1C3 vector.
This part is a part of a device patented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use.
                                    


                                    
                                    
                                        GFP-AID