Team:Groningen/Template/MODULE/Notebook/characterisation/week2

From 2014.igem.org

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<div class="listing">
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<div class="item">Transformed both, pSB1C3 containing the NisA BioBrick and pSB1C3 with the Superfolded GFP Biobrick to two different <i>E.col</i> DH5α strains</div>
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<div class="item">Transformed both, pSB1C3 containing the NisA BioBrick and pSB1C3 with the Superfolded GFP Biobrick to two different <i>E. coli</i> DH5α strains</div>
<div class="item">The transformations were checked on gel, and the desired bands were observed, therefore the transformation was most likely successful</div>
<div class="item">The transformations were checked on gel, and the desired bands were observed, therefore the transformation was most likely successful</div>
<div class="item">The plasmids with the desired BioBricks were isolated out of <i>E. coli</i> DH5α, and glycerol stocks were made of the two strains containing the previous named parts</div>
<div class="item">The plasmids with the desired BioBricks were isolated out of <i>E. coli</i> DH5α, and glycerol stocks were made of the two strains containing the previous named parts</div>

Revision as of 16:44, 17 October 2014

8 - 14 September
 
Obtaining the NisA (BBa_K1365000) and Superfolded GFP (BBa_K1365020) BioBrick in larger concentration for further use
 
Transformed both, pSB1C3 containing the NisA BioBrick and pSB1C3 with the Superfolded GFP Biobrick to two different E. coli DH5α strains
The transformations were checked on gel, and the desired bands were observed, therefore the transformation was most likely successful
The plasmids with the desired BioBricks were isolated out of E. coli DH5α, and glycerol stocks were made of the two strains containing the previous named parts