Team:Cooper Union/Notebook/Biohack May

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<a href="https://2014.igem.org/Team:Cooper_Union" span="The Cooper Union: μToolbox"><img src="https://static.igem.org/mediawiki/2014/b/bd/CU_2014_logo.png" height="150"  /></a></div>
 
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<a href="https://2014.igem.org/Team:Cooper_Union/Project" span="Projects"><img src="https://static.igem.org/mediawiki/2014/2/27/CU_2014_LogoP.png" height="100" style="vertical-align: top;" title="Projects" /></a>
 
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<a href="https://2014.igem.org/Team:Cooper_Union/Safety" span="Safety"><img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Safety" /></a>
 
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<a href="https://2014.igem.org/Team:Cooper_Union/Outreach" span="Outreach"><img src="https://static.igem.org/mediawiki/2014/7/74/CU_2014_logoO.png" height="100" style="vertical-align: top;" title="Outreach" /></a>
 
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<a href="https://2014.igem.org/Team:Cooper_Union/Notebook" span="Notebook"><img src="https://static.igem.org/mediawiki/2014/d/da/CU_2014_LogoN.png" height="100" style="vertical-align: top;" title="Notebook" /></a>
 
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<a href="https://2014.igem.org/Team:Cooper_Union/biofanfic" span="Team"><img src="https://static.igem.org/mediawiki/2014/3/38/CU_2014_LogoT.png" height="100" style="vertical-align: top;" title="Team" /></a></div>
 
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<a href="http://www.cooper.edu" target="_blank" span="The Cooper Union"><img src="https://static.igem.org/mediawiki/2014/9/90/CU_2014_Cooper_union_logo.png" height="150" /></a>
 
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        <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" >
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            <span>Notebook</span>
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        <a href="http://www.cooper.edu" target="_blank" span="The Cooper Union"><img src="https://static.igem.org/mediawiki/2014/9/90/CU_2014_Cooper_union_logo.png" height="150" /></a>
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Revision as of 16:33, 17 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL 




5/19/14
Calibrated Nanodrop.
Nanodropped all of our samples. Very low concentration of DNA.
Ordered primers to PCR our g-blocks.

5/22/14:
Struck out plates with pACYC184 with spectinomycin to grow it.
Digested pBR322 with HindIII.
Double digested pBR322 with HindIII/EcoRI.

5/23/14
Ran gel of digests from 5/22/14. Everything as it should be.
Purified the digested plasmids. 
Nanodropped plasmids; results:
	Single digest I: 52.7ng/uL
	Single digest II: 64.5ng/uL
	Double digest I: 26.3ng/uL
	Double digest II: 41.9ng/uL
Phosphatase treated the four digested plasmids. 

5/27/14
Purified phosphatase treated plasmids from 5/23/14. 
Nanodropped the purified plasmids; results:
	Single digest I: 43.4ng/uL
	Single digest II: 53.6ng/uL
	Double digest I: 43.7ng/uL
	Double digest II: 16.5ng/uL

5/28/14



Projects Safety Outreach Notebook Team

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