Team:Cooper Union/Notebook/Biohack April

From 2014.igem.org

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<div class="cu-footer">
 
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<a href="https://2014.igem.org/Team:Cooper_Union" span="The Cooper Union: μToolbox"><img src="https://static.igem.org/mediawiki/2014/b/bd/CU_2014_logo.png" height="150"  /></a></div>
 
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<div class="bottom-center">
 
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<a href="https://2014.igem.org/Team:Cooper_Union/Project" span="Projects"><img src="https://static.igem.org/mediawiki/2014/2/27/CU_2014_LogoP.png" height="100" style="vertical-align: top;" title="Projects" /></a>
 
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<a href="https://2014.igem.org/Team:Cooper_Union/Safety" span="Safety"><img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Safety" /></a>
 
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<a href="https://2014.igem.org/Team:Cooper_Union/Outreach" span="Outreach"><img src="https://static.igem.org/mediawiki/2014/7/74/CU_2014_logoO.png" height="100" style="vertical-align: top;" title="Outreach" /></a>
 
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<a href="https://2014.igem.org/Team:Cooper_Union/Notebook" span="Notebook"><img src="https://static.igem.org/mediawiki/2014/d/da/CU_2014_LogoN.png" height="100" style="vertical-align: top;" title="Notebook" /></a>
 
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<a href="https://2014.igem.org/Team:Cooper_Union/biofanfic" span="Team"><img src="https://static.igem.org/mediawiki/2014/3/38/CU_2014_LogoT.png" height="100" style="vertical-align: top;" title="Team" /></a></div>
 
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<div class="bottom-right">
 
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<a href="http://www.cooper.edu" target="_blank" span="The Cooper Union"><img src="https://static.igem.org/mediawiki/2014/9/90/CU_2014_Cooper_union_logo.png" height="150" /></a>
 
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</div>
 
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<div class="cu-footer">
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    <div class="bottom-left">
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        <a href="https://2014.igem.org/Team:Cooper_Union" span="The Cooper Union: μToolbox"><img src="https://static.igem.org/mediawiki/2014/b/bd/CU_2014_logo.png" height="150"  /></a></div>
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    <div class="bottom-center">
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        <a href="https://2014.igem.org/Team:Cooper_Union/Project" class="black" >
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            <img src="https://static.igem.org/mediawiki/2014/2/27/CU_2014_LogoP.png" height="100" style="vertical-align: top;" title="Projects" />
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            <span>Projects</span>
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        </a>
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        <a href="https://2014.igem.org/Team:Cooper_Union/Safety" class="red" >
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            <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Safety" />
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            <span>Safety</span>
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        </a>
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        <a href="https://2014.igem.org/Team:Cooper_Union/Outreach"  class="yellow">
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            <img src="https://static.igem.org/mediawiki/2014/7/74/CU_2014_logoO.png" height="100" style="vertical-align: top;" title="Outreach" />
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            <span>Outreach</span>
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        </a>
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        <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" >
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            <img src="https://static.igem.org/mediawiki/2014/d/da/CU_2014_LogoN.png" height="100" style="vertical-align: top;" title="Notebook" />
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            <span>Notebook</span>
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        </a>
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        <a href="https://2014.igem.org/Team:Cooper_Union/biofanfic"class="blue">
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            <img src="https://static.igem.org/mediawiki/2014/3/38/CU_2014_LogoT.png" height="100" style="vertical-align: top;" title="Team" />
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            <span>Team</span>
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        </a>
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    </div>
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    <div class="bottom-right">
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        <a href="http://www.cooper.edu" target="_blank" span="The Cooper Union"><img src="https://static.igem.org/mediawiki/2014/9/90/CU_2014_Cooper_union_logo.png" height="150" /></a>
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    </div>
</div>
</div>

Revision as of 16:33, 17 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL


3/13/14

  • Digested pBR322 with HindIII.
  • Double digested pBR322 with HindIII and EcoRI.

3/27/14

  • Ran digests from 3/13/14 on gel.
  • Excised gel fragments with plasmid in them.
  • Purified gel fragments.

4/10/14

Phosphatase treated the digested and double digested pBR322 from 3/13/14. 4/17/14
  • Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI.
  • Performed five ligations:
    pBR322 with UV
    pBR322 with HSP
    pBR322 with GFP
    UV with GFP
    HSP with GFP

4/24/14

  • Purified UV+GFP and HSP+GFP ligations.
  • Digested the two ligations with HindIII.
  • Ligated each one into pBR322.


Projects Safety Outreach Notebook Team

Retrieved from "http://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_April"