Team:Penn State/CodonOptimization

From 2014.igem.org

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<p><center><h3>Codon Optimization: Engineering a More Useful Gene at the Codon Level</h3></center></p>
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<p><h2>Project Summary</h2></p>
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<p>Codons are groups of three nucleotides that specify a single amino acid, which is then added to a growing polypeptide chain during translation. Even though each codon spefifies only one amino acid, some amino acids are coded by multiple codons. It has been demonstrated that the genome of <i>E.coli</i> shows statistical preference for some of these degenerate codons over others, and it is hypothesized that these codons translate more efficiently than non preferred degenerate codons. We constructed synthetic reporter genes entirely from codons hypothesized to be fast or slow,and characterized them in <i>E.coli</i>, demonstrating that...  </p>
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Revision as of 19:54, 14 July 2014



WELCOME TO PENN STATE iGEM 2014!

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CODON OPTIMIZATION PROJECT

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Project Description

tell about project - give background, essentially write the abstract. (1-2 paragraphs)

references

iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.

It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.

INFO TO INCLUDE:

  1. Overall project summary
  2. Project Details
  3. Materials and Methods
  4. The Experiments
  5. Results
  6. Data analysis
  7. Conclusions

Click HERE to return to the Projects page.

Codon Optimization: Engineering a More Useful Gene at the Codon Level

Project Summary

Codons are groups of three nucleotides that specify a single amino acid, which is then added to a growing polypeptide chain during translation. Even though each codon spefifies only one amino acid, some amino acids are coded by multiple codons. It has been demonstrated that the genome of E.coli shows statistical preference for some of these degenerate codons over others, and it is hypothesized that these codons translate more efficiently than non preferred degenerate codons. We constructed synthetic reporter genes entirely from codons hypothesized to be fast or slow,and characterized them in E.coli, demonstrating that...

Why is this important?

Background

Our Objectives

1) Objective 1

2) Objective 2

3) Objective 3

4) Objective 4

5) Objective 5

Design Methods

The figure below...

Figure Heading

Caption

Bold heading:

Retrieved from "http://2014.igem.org/Team:Penn_State/CodonOptimization"