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Team:Evry/Notebook/Transposons/11.09.14
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Revision as of 16:27, 17 October 2014
A stock of the cells DH5α pir with pNK2 is performed.
The plasmid pNK2 is purified from precultur of DH5α pir transformed. A kit of Macherey-Nagel of Plasmid DNA purification is used.
DH5α pir transformed is sowed in LB kanamycin and one glycerol is making.
