Team:Cambridge-JIC/Guide/Transformation

From 2014.igem.org

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=Transformation=
=Transformation=
This section contains the information for transforming Marchantia spores. The best established procedures take around 7 days.
This section contains the information for transforming Marchantia spores. The best established procedures take around 7 days.
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==Links==
==Links==
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The steps in transformation are:
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The steps in transformation are roughly:
1. [[Team:Cambridge-JIC/Guide/Transformation/TransformingAgro | Electroporate Agrobacterium]]
1. [[Team:Cambridge-JIC/Guide/Transformation/TransformingAgro | Electroporate Agrobacterium]]
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4. [[Team:Cambridge-JIC/Guide/Transformation/SelectingTransformants| Select transformants]]
4. [[Team:Cambridge-JIC/Guide/Transformation/SelectingTransformants| Select transformants]]
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Latest revision as of 15:38, 17 October 2014

Cambridge iGEM 2014


Transformation

This section contains the information for transforming Marchantia spores. The best established procedures take around 7 days.

See the outline for an overview of the process.

Links

The steps in transformation are roughly:

1. Electroporate Agrobacterium

2. Prepare spores

3. Cocultivate or use AgarTrap

4. Select transformants