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Team:Tuebingen/Notebook/Protocols/LB agar
From 2014.igem.org
(Difference between revisions)
| Line 41: | Line 41: | ||
<ol> | <ol> | ||
| - | <li> Add the regents to 800ml | + | <li> Add the regents to 800ml H<sub>2</sub>O</li> |
<li> Adjust pH to 7.5 with NaOH</li> | <li> Adjust pH to 7.5 with NaOH</li> | ||
| Line 49: | Line 49: | ||
<li> Melt agar into solution in the microwave</li> | <li> Melt agar into solution in the microwave</li> | ||
| - | <li> Adjust volume to 1L with | + | <li> Adjust volume to 1L with dH<sub>2</sub>O</li> |
<li> Sterilize by autoclaving</li> | <li> Sterilize by autoclaving</li> | ||
Revision as of 15:32, 17 October 2014
Protocols
LB agar
Reagents
| 10 g | Bacto-tryptone |
| 5 g | yeast extract |
| 10 g | NaCl |
Procedure
- Add the regents to 800ml H2O
- Adjust pH to 7.5 with NaOH
- Add 15g agar
- Melt agar into solution in the microwave
- Adjust volume to 1L with dH2O
- Sterilize by autoclaving
