Team:Cooper Union/Notebook/Biohack April

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Revision as of 15:32, 17 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL


3/13/14

  • Digested pBR322 with HindIII.
  • Double digested pBR322 with HindIII and EcoRI.

3/27/14

  • Ran digests from 3/13/14 on gel.
  • Excised gel fragments with plasmid in them.
  • Purified gel fragments.

4/10/14

Phosphatase treated the digested and double digested pBR322 from 3/13/14. 4/17/14
  • Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI.
  • Performed five ligations:
    pBR322 with UV
    pBR322 with HSP
    pBR322 with GFP
    UV with GFP
    HSP with GFP

4/24/14

  • Purified UV+GFP and HSP+GFP ligations.
  • Digested the two ligations with HindIII.
  • Ligated each one into pBR322.


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