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Team:Tuebingen/Notebook/Protocols/TBE buffer
From 2014.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
<div id="TueContent"> | <div id="TueContent"> | ||
| - | + | <h1>Protocols</h1> | |
<h3>TBE buffer</h3> | <h3>TBE buffer</h3> | ||
<h4>Reagents</h4> | <h4>Reagents</h4> | ||
| Line 25: | Line 25: | ||
<tr> | <tr> | ||
<td style="text-align: center">0.85 M</td> | <td style="text-align: center">0.85 M</td> | ||
| - | <td>B(OH)3</td> | + | <td>B(OH)<sub>3</sub></td> |
</tr> | </tr> | ||
| Line 37: | Line 37: | ||
<h4>Procedure</h4> | <h4>Procedure</h4> | ||
| - | <p>Add the reagents to 1000 ml | + | <p>Add the reagents to 1000 ml H<sub>2</sub>0. Adjust to pH 8.3 with B(OH)<sub>3</sub>.</p> |
Latest revision as of 15:28, 17 October 2014
Protocols
TBE buffer
Reagents
| 1 M | Tris |
| 0.85 M | B(OH)3 |
| 1 M | EDTA 0.5 M pH 8.0 |
Procedure
Add the reagents to 1000 ml H20. Adjust to pH 8.3 with B(OH)3.
