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Team:Cambridge-JIC/Guide/Constructs/Terminators
From 2014.igem.org
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Unfortunately, there is a lack of terminators in MP. The only one to find consistent use is the [http://parts.igem.org/Part:BBa_K1484215 nopaline synthase terminator]. | Unfortunately, there is a lack of terminators in MP. The only one to find consistent use is the [http://parts.igem.org/Part:BBa_K1484215 nopaline synthase terminator]. | ||
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This makes sequencing multi-gene constructs difficult. Slight variations to the sequence can be introduced via PCR, and mutiple repeats of the terminator can be added. | This makes sequencing multi-gene constructs difficult. Slight variations to the sequence can be introduced via PCR, and mutiple repeats of the terminator can be added. | ||
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Latest revision as of 15:26, 17 October 2014
Unfortunately, there is a lack of terminators in MP. The only one to find consistent use is the [http://parts.igem.org/Part:BBa_K1484215 nopaline synthase terminator].
This is a terminator found on the wild-type A. tumefaciens T-DNA!
This makes sequencing multi-gene constructs difficult. Slight variations to the sequence can be introduced via PCR, and mutiple repeats of the terminator can be added.
