Team:NCTU Formosa/Notebook/test7

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Revision as of 15:05, 17 October 2014

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10/3

Cultivation of

[P_cons+RBS+NdeI]Cr

[P_cons+RBS+NcoI]Cr

[KpnI-1]Cr

[NdeI-1]Cr

[XhoI-1]Cr

[SacI-1]Cr

[AseI-1]Cr

[ P_cons+RBS+HindIII-1]Cr

[ P_cons+RBS+KpnI-4]Cr

[ P_cons+RBS+AseI-5]Cr

[ P_cons+RBS+XhoI-1]Cr

[ P_cons+RBS+SacI-1]Cr

[ P_cons+RBS+KpnI-1+J61048]Cr

[SalI-4]Cr

[P_cons+RBS+SalI-3+J61048]Cr

[HindIII-4]Cr

[P_cons+RBS+AseI+J61048]Cr

[NheI]

[NcoI]

Electrophoresis of

[NdeI](EP)

[NcoI](EP)

[SacI](EP)

Transformation of

[P_cons+RBS+NdeI]Cr

[P_cons+RBS+SacI]Cr

DNA Sequencing of

[P_cons+RBS+NdeI+J61048-1]

[P_cons+RBS+HindIII+J61048-1]

[P_cons+RBS+SalI+J61048-4]

[P_cons+RBS+SacI+J61048-1]

[P_cons+RBS+SacI+J61048-1](fail)

10/4

Digestion of

[P_cons+RBS+XhoI+J61048-2, -4, -5]

[P_cons+RBS+SacI+J61048-10, -11]

[P_cons+RBS+NheI+J61048-20]

[P_cons+RBS+NheI]Cr

[P_cons+RBS+NcoI]Cr

Cultivation of

[P_cons+RBS+NheI-1, -2, -4, -6, -10, -12]

[P_cons+RBS+SalI-1, -3, -6, -7, -11, -12]

Cultivation of

[P_cons+RBS+NcoI+J61048-1~8]

PCR of

[P_cons+RBS+NheI-1, -2, -4, -6, -10, -12]

[P_cons+RBS+SacI-1, -3, -6, -7, -11, -12]

[P_cons+RBS+NcoI+J61048-1~8]

Ligation of

[P_cons+RBS+NheI]Kr

[P_cons+RBS+NcoI]Kr

Electrophoresis of

[XhoI-2, -4, -5](dig)

[SacI-10, -11](dig)

[NheI-20](dig)

[P_cons+RBS+NdeI](dig)

[P_cons+RBS+NcoI](dig)

[PCR NcoI-1~8]

Mini-prep of

[P_cons+RBS+NheI-6]

[P_cons+RBS+SalI-1, -7, -11]

Digestion of

[P_cons+RBS+NheI-6](EP)

[P_cons+RBS+SalI-1, -7, -11](EP)

[P_cons+RBS+NcoI]

[P_cons+RBS+Ndel]

PCR of and cultivation of

[P_cons+RBS+NcoI+J61048]

Mini-prep of

[P_cons+RBS+NcoI+J61048-7]Cr

Electrophoresis of

[P_cons+RBS+KphI+J61048-1]Cr

[P_cons+RBS+SalI+J61048-3]Cr

[P_cons+RBS+AseI+J61048 ]Cr

[P_cons+RBS+NdeI+J61048-1]

[P_cons+RBS+HindIII+J61048-1]

[P_cons+RBS+SacI+J61048 -1]

[P_cons+RBS+NcoI+J61048](dig)

[SacI-11]

DNA Sequencing of

[P_cons+RBS+NdeI]-2 Cr

[P_cons+RBS+NcoI]-2 Cr

[P_cons+RBS+NheI]-6 Cr

[P_cons+RBS+SalI-1]Cr

[P_cons+RBS+SalI-11]Cr

[P_cons+RBS+HindIII-1]Cr

[NcoI-12]

[P_cons+RBS+KpnI-1+J61048]Cr

[P_cons+RBS+AseI+J61048]Cr

[P_cons+RBS+SalI-3+J61048]Cr

[P_cons+RBS+HindIII-1+J61048]Cr

[P_cons+RBS+XhoI-2+J61048]Cr

[P_cons+RBS+XhoI-5+J61048]Cr

[P_cons+RBS+NcoI-5+J61048]Cr

[P_cons+RBS+SacI-11-1+J61048]Cr

[P_cons+RBS+NdeI-1+J61048]Cr

[P_cons+RBS+NheI-20+J61048]Cr

[P_cons+RBS+AseI-7+J61048]Cr

[P_cons+RBS+HindIII-7+J61048]Ar

[P_cons+RBS+SacI-16+J61048]Ar

[P_cons+RBS+SalI-17+J61048]Ar

[P_cons+RBS+NdeI-2+J61048]Cr

[P_cons+RBS+NcoI-2+J61048]Cr

[P_cons+RBS+XhoI+J61048]Ar

[P_cons+RBS+KpnI-7+J61048]Ar

[P_cons+RBS+NheI-11+J61048]Ar

10/7

PCR of

[P_cons+RBS+NheI+J61048]

[P_cons+RBS+SalI in PSB3C5]

[P_cons+RBS+SacI in PSB3C5]

Cultivation of

[P_cons+RBS+NheI+J61048-1, -3, -4, -6, -8, -10, -15, -16]

Transformation of

[P_cons+RBS+XhoI-2 +J61048]

[P_cons+RBS+XhoI-5 +J61048-1]

[P_cons+RBS+SacI-11+J61048]

PCR of

[P_cons+RBS+ NdeI in PSB3C5]

[P_cons+RBS+KpnI in PSB3C5]

[P_cons+RBS+AseI in PSB3C5]

[P_cons+RBS+XhoI in PSB3C5]

[P_cons+RBS+NcoI in PSB3C5]

[P_cons+RBS+NheI in PSB3C5]

nine kinds of PBAN

DNA Sequencing of

[P_cons+RBS+NcoI-5+J61048]

10/8

DNA Sequencing of

[PBAN NcoI]

[P_cons+RBS+SalI -1]

[P_cons+RBS+SalI-11]

[P_cons+RBS+NheI-6]

[P_cons+RBS+xhol-2+RBS+BFP+TER]

[P_cons+RBS+SacI-11+RBS+BFP+TER]

[P_cons+RBS+ Nhel-20+RBS+BFP+TER]

Ligation of

P_cons+RBS+NcoI on psb3c5

10/9

ALL Nhel-9(10/7)x2

ALL SacI-11(10/7)x1

ALL xhol-2(10/7)x1

Electrophoresis of

Gel 1

Well1 Marker

Well2 P_cons+RBS+NheI-20+RBS+BFP+TER

Well3 P_cons+RBS+Hind-1+RBS+BFP+TER

Well4 P_cons+RBS+KpnI-1+RBS+BFP+TER

Well5 P_cons+RBS+Ndel-1+RBS+BFP+TER

2 Well6 P_cons+RBS+NcoI-5+RBS+BFP+TER

Well7 P_cons+RBS+AseI ALL

Well8 PBAN NcoI-12

Well9 P_cons+RBS+NheI-6

Well10 P_cons+RBS+Hind-1

Well11 P_cons+RBS+NcoI-20

Well12 P_cons+RBS+NcoI-2-2

Well13 P_cons+RBS+Ndel-2-1

Well14 P_cons+RBS+Nedl-2-2

Well15 P_cons+RBS+SacI-1

Well16 P_cons+RBS+SacI-11

Well17 Marker

Gel 2

Well1 marker

Well2 P_cons+RBS+NheI-11

Well3 P_cons+RBS+Hind3

Well4 P_cons+RBS+KpnI-7

Well5 P_cons+RBS+SacI-16

Well6 P_cons+RBS+SalI-12

Well7 P_cons+RBS+Xhol

Transformation of

P_cons+RBS+SacI-3 on psb3c5

P_cons+RBS+KpnI-1 on psb3c5

P_cons+RBS+NcoI-1 on psb3c5

Mini-prep of and sequencing of

ALL NheI-2 x1

ALL SacI-11 x1

ALL Xhol-2 X1

10/10

Transformation of

P_cons+RBS+Ndel on psb3c5

P_cons+RBS+NcoI on psb3c5

Electrophoresis of

Well1 marker

Well2 P_cons+RBS+SacI-3 on psb3c5

Well3 P_cons+RBS+KpnI-1 on psb3c5

Well4 P_cons+RBS+NcoI-1 on psb3c5

10/12

PCR of and Electrophoresis of

Well1 P_cons+RBS+NcoI+psb3c5

Well2 P_cons+RBS+Ndel+psb3c5

None

@@ Line 76: / Line 76: @@
 <font color="#FF0000"><span style="font-size:1cm;">10/3</span></font><br></p><p>
 <font color="yellow">Cultivation of</font></p><p>
-[Pcons+RBS+NdeI]Cr</p><p>
+[P<sub>cons</sub>+RBS+NdeI]Cr</p><p>
-[Pcons+RBS+NcoI]Cr</p><p>
+[P<sub>cons</sub>+RBS+NcoI]Cr</p><p>
 [KpnI-1]Cr</p><p>
   [NdeI-1]Cr</p><p>
@@ Line 83: / Line 83: @@
   [SacI-1]Cr</p><p>
 [AseI-1]Cr</p><p>
-[ Pcons+RBS+HindIII-1]Cr</p><p>
+[ P<sub>cons</sub>+RBS+HindIII-1]Cr</p><p>
-  [ Pcons+RBS+KpnI-4]Cr</p><p>
+  [ P<sub>cons</sub>+RBS+KpnI-4]Cr</p><p>
-  [ Pcons+RBS+AseI-5]Cr</p><p>
+  [ P<sub>cons</sub>+RBS+AseI-5]Cr</p><p>
-[ Pcons+RBS+XhoI-1]Cr</p><p>
+[ P<sub>cons</sub>+RBS+XhoI-1]Cr</p><p>
-  [ Pcons+RBS+SacI-1]Cr</p><p>
+  [ P<sub>cons</sub>+RBS+SacI-1]Cr</p><p>
-[ Pcons+RBS+KpnI-1+J61048]Cr</p><p>
+[ P<sub>cons</sub>+RBS+KpnI-1+J61048]Cr</p><p>
   [SalI-4]Cr</p><p>
-[Pcons+RBS+SalI-3+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+SalI-3+J61048]Cr</p><p>
 [HindIII-4]Cr</p><p>
-[Pcons+RBS+AseI+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+AseI+J61048]Cr</p><p>
   [NheI]</p><p>
   [NcoI]</p><p>
@@ Line 100: / Line 100: @@
   [SacI](EP)</p><p>
 <font color="yellow">Transformation of</font></p><p>
-  [Pcons+RBS+NdeI]Cr</p><p>
+  [P<sub>cons</sub>+RBS+NdeI]Cr</p><p>
-  [Pcons+RBS+SacI]Cr</p><p>
+  [P<sub>cons</sub>+RBS+SacI]Cr</p><p>
 <font color="yellow">DNA Sequencing of</font></p><p>
-  [Pcons+RBS+NdeI+J61048-1]</p><p>
+  [P<sub>cons</sub>+RBS+NdeI+J61048-1]</p><p>
-  [ Pcons+RBS+HindIII+J61048-1]</p><p>
+  [P<sub>cons</sub>+RBS+HindIII+J61048-1]</p><p>
-  [ Pcons+RBS+SalI+J61048-4]</p><p>
+  [P<sub>cons</sub>+RBS+SalI+J61048-4]</p><p>
-  [Pcons+RBS+SacI+J61048-1]</p><p>
+  [P<sub>cons</sub>+RBS+SacI+J61048-1]</p><p>
-[Pcons+RBS+SacI+J61048-1](fail)</p><p>
+[P<sub>cons</sub>+RBS+SacI+J61048-1](fail)</p><p>
 <font color="#FF0000"><span style="font-size:1cm;">10/4</span></font><br></p><p>
 <font color="yellow">Digestion of</font></p><p>
-  [Pcons+RBS+XhoI+J61048-2, -4, -5] </p><p>
+  [P<sub>cons</sub>+RBS+XhoI+J61048-2, -4, -5] </p><p>
-  [Pcons+RBS+SacI+J61048-10, -11]</p><p>
+  [P<sub>cons</sub>+RBS+SacI+J61048-10, -11]</p><p>
-[Pcons+RBS+NheI+J61048-20]</p><p>
+[P<sub>cons</sub>+RBS+NheI+J61048-20]</p><p>
-  [Pcons+RBS+NheI]Cr</p><p>
+  [P<sub>cons</sub>+RBS+NheI]Cr</p><p>
-  [Pcons+RBS+NcoI]Cr</p><p>
+  [P<sub>cons</sub>+RBS+NcoI]Cr</p><p>
 <font color="yellow">Cultivation of </font></p><p>
-[Pcons+RBS+NheI-1, -2, -4, -6, -10, -12]</p><p>
+[P<sub>cons</sub>+RBS+NheI-1, -2, -4, -6, -10, -12]</p><p>
-  [Pcons+RBS+SalI-1, -3, -6, -7, -11, -12] </p><p>
+  [P<sub>cons</sub>+RBS+SalI-1, -3, -6, -7, -11, -12] </p><p>
 <font color="yellow">Cultivation of </font></p><p>
-[Pcons+RBS+NcoI+J61048-1~8]</p><p>
+[P<sub>cons</sub>+RBS+NcoI+J61048-1~8]</p><p>
 <font color="yellow">PCR of</font></p><p>
-  [Pcons+RBS+NheI-1, -2, -4, -6, -10, -12]</p><p>
+  [P<sub>cons</sub>+RBS+NheI-1, -2, -4, -6, -10, -12]</p><p>
-  [Pcons+RBS+SacI-1, -3, -6, -7, -11, -12]</p><p>
+  [P<sub>cons</sub>+RBS+SacI-1, -3, -6, -7, -11, -12]</p><p>
-  [Pcons+RBS+NcoI+J61048-1~8]</p><p>
+  [P<sub>cons</sub>+RBS+NcoI+J61048-1~8]</p><p>
 <font color="yellow">Ligation of </font></p><p>
-[Pcons+RBS+NheI]Kr</p><p>
+[P<sub>cons</sub>+RBS+NheI]Kr</p><p>
-  [ Pcons+RBS+NcoI]Kr</p><p>
+  [P<sub>cons</sub>+RBS+NcoI]Kr</p><p>
 <font color="yellow">Electrophoresis of </font></p><p>
 [XhoI-2, -4, -5](dig)</p><p>
   [SacI-10, -11](dig)</p><p>
   [NheI-20](dig)</p><p>
-  [Pcons+RBS+NdeI](dig)</p><p>
+  [P<sub>cons</sub>+RBS+NdeI](dig)</p><p>
-  [Pcons+RBS+NcoI](dig)</p><p>
+  [P<sub>cons</sub>+RBS+NcoI](dig)</p><p>
   [PCR NcoI-1~8]</p><p>
 <font color="yellow">Mini-prep of</font></p><p>
-  [Pcons+RBS+NheI-6]</p><p>
+  [P<sub>cons</sub>+RBS+NheI-6]</p><p>
-  [Pcons+RBS+SalI-1, -7, -11]</p><p>
+  [P<sub>cons</sub>+RBS+SalI-1, -7, -11]</p><p>
 <font color="yellow">Digestion of </font></p><p>
-[Pcons+RBS+NheI-6](EP)</p><p>
+[P<sub>cons</sub>+RBS+NheI-6](EP)</p><p>
-  [Pcons+RBS+SalI-1, -7, -11](EP)</p><p>
+  [P<sub>cons</sub>+RBS+SalI-1, -7, -11](EP)</p><p>
-  [Pcons+RBS+NcoI]</p><p>
+  [P<sub>cons</sub>+RBS+NcoI]</p><p>
-  [Pcons+RBS+Ndel]</p><p>
+  [P<sub>cons</sub>+RBS+Ndel]</p><p>
 <font color="yellow">PCR of and cultivation of </font></p><p>
-[Pcons+RBS+NcoI+J61048]</p><p>
+[P<sub>cons</sub>+RBS+NcoI+J61048]</p><p>
 <font color="yellow">Mini-prep of</font></p><p>
-  [Pcons+RBS+NcoI+J61048-7]Cr</p><p>
+  [P<sub>cons</sub>+RBS+NcoI+J61048-7]Cr</p><p>
 <font color="yellow">Electrophoresis of </font></p><p>
-[Pcons+RBS+KphI+J61048-1]Cr</p><p>
+[P<sub>cons</sub>+RBS+KphI+J61048-1]Cr</p><p>
-  [ Pcons+RBS+SalI+J61048-3]Cr</p><p>
+  [P<sub>cons</sub>+RBS+SalI+J61048-3]Cr</p><p>
-  [ Pcons+RBS+AseI+J61048 ]Cr</p><p>
+  [P<sub>cons</sub>+RBS+AseI+J61048 ]Cr</p><p>
-  [Pcons+RBS+NdeI+J61048-1]</p><p>
+  [P<sub>cons</sub>+RBS+NdeI+J61048-1]</p><p>
-  [ Pcons+RBS+HindIII+J61048-1]</p><p>
+  [P<sub>cons</sub>+RBS+HindIII+J61048-1]</p><p>
-  [ Pcons+RBS+SacI+J61048 -1]</p><p>
+  [P<sub>cons</sub>+RBS+SacI+J61048 -1]</p><p>
-  [Pcons+RBS+NcoI+J61048](dig)</p><p>
+  [P<sub>cons</sub>+RBS+NcoI+J61048](dig)</p><p>
   [SacI-11]</p><p>
 <font color="yellow">DNA Sequencing of </font></p><p>
-[Pcons+RBS+NdeI]-2 Cr</p><p>
+[P<sub>cons</sub>+RBS+NdeI]-2 Cr</p><p>
-[Pcons+RBS+NcoI]-2 Cr</p><p>
+[P<sub>cons</sub>+RBS+NcoI]-2 Cr</p><p>
-[Pcons+RBS+NheI]-6 Cr</p><p>
+[P<sub>cons</sub>+RBS+NheI]-6 Cr</p><p>
-[Pcons+RBS+SalI-1]Cr</p><p>
+[P<sub>cons</sub>+RBS+SalI-1]Cr</p><p>
-[Pcons+RBS+SalI-11]Cr</p><p>
+[P<sub>cons</sub>+RBS+SalI-11]Cr</p><p>
-[Pcons+RBS+HindIII-1]Cr</p><p>
+[P<sub>cons</sub>+RBS+HindIII-1]Cr</p><p>
 [NcoI-12]</p><p>
-[ Pcons+RBS+KpnI-1+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+KpnI-1+J61048]Cr</p><p>
-[ Pcons+RBS+AseI+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+AseI+J61048]Cr</p><p>
-[ Pcons+RBS+SalI-3+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+SalI-3+J61048]Cr</p><p>
-[ Pcons+RBS+HindIII-1+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+HindIII-1+J61048]Cr</p><p>
-[ Pcons+RBS+XhoI-2+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+XhoI-2+J61048]Cr</p><p>
-[ Pcons+RBS+XhoI-5+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+XhoI-5+J61048]Cr</p><p>
-[ Pcons+RBS+NcoI-5+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+NcoI-5+J61048]Cr</p><p>
-[ Pcons+RBS+SacI-11-1+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+SacI-11-1+J61048]Cr</p><p>
-[ Pcons+RBS+NdeI-1+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+NdeI-1+J61048]Cr</p><p>
-[ Pcons+RBS+NheI-20+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+NheI-20+J61048]Cr</p><p>
-[ Pcons+RBS+AseI-7+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+AseI-7+J61048]Cr</p><p>
-[ Pcons+RBS+HindIII-7+J61048]Ar</p><p>
+[P<sub>cons</sub>+RBS+HindIII-7+J61048]Ar</p><p>
-[ Pcons+RBS+SacI-16+J61048]Ar</p><p>
+[P<sub>cons</sub>+RBS+SacI-16+J61048]Ar</p><p>
-[ Pcons+RBS+SalI-17+J61048]Ar</p><p>
+[P<sub>cons</sub>+RBS+SalI-17+J61048]Ar</p><p>
-[ Pcons+RBS+NdeI-2+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+NdeI-2+J61048]Cr</p><p>
-[ Pcons+RBS+NcoI-2+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+NcoI-2+J61048]Cr</p><p>
-[ Pcons+RBS+XhoI+J61048]Ar</p><p>
+[P<sub>cons</sub>+RBS+XhoI+J61048]Ar</p><p>
-[ Pcons+RBS+KpnI-7+J61048]Ar</p><p>
+[P<sub>cons</sub>+RBS+KpnI-7+J61048]Ar</p><p>
-[ Pcons+RBS+NheI-11+J61048]Ar</p>
+[P<sub>cons</sub>+RBS+NheI-11+J61048]Ar</p>
 <br></div>
 <div class="single note">
@@ Line 188: / Line 188: @@
 <font color="#FF0000"><span style="font-size:1cm;">10/7</span></font><br></p><p>
 <font color="yellow">PCR of</font></p><p>
-  [ Pcons+RBS+NheI+J61048]</p><p>
+  [P<sub>cons</sub>+RBS+NheI+J61048]</p><p>
-[ Pcons+RBS+SalI in PSB3C5]</p><p>
+[P<sub>cons</sub>+RBS+SalI in PSB3C5]</p><p>
-  [Pcons+RBS+SacI in PSB3C5]</p><p>
+  [P<sub>cons</sub>+RBS+SacI in PSB3C5]</p><p>
 <font color="yellow">Cultivation of</font> </p><p>
-[Pcons+RBS+NheI+J61048-1, -3, -4, -6, -8, -10, -15, -16]</p><p>
+[P<sub>cons</sub>+RBS+NheI+J61048-1, -3, -4, -6, -8, -10, -15, -16]</p><p>
 <font color="yellow">Transformation of </font></p><p>
-[Pcons+RBS+XhoI-2 +J61048]</p><p>
+[P<sub>cons</sub>+RBS+XhoI-2 +J61048]</p><p>
-  [Pcons+RBS+XhoI-5 +J61048-1]</p><p>
+  [P<sub>cons</sub>+RBS+XhoI-5 +J61048-1]</p><p>
-  [Pcons+RBS+SacI-11+J61048]</p><p>
+  [P<sub>cons</sub>+RBS+SacI-11+J61048]</p><p>
 <font color="yellow">PCR of</font></p><p>
-   [Pcons+RBS+ NdeI  in PSB3C5]</p><p>
+   [P<sub>cons</sub>+RBS+ NdeI  in PSB3C5]</p><p>
-  [Pcons+RBS+KpnI in PSB3C5]</p><p>
+  [P<sub>cons</sub>+RBS+KpnI in PSB3C5]</p><p>
-  [Pcons+RBS+AseI in PSB3C5]</p><p>
+  [P<sub>cons</sub>+RBS+AseI in PSB3C5]</p><p>
-  [Pcons+RBS+XhoI in PSB3C5]</p><p>
+  [P<sub>cons</sub>+RBS+XhoI in PSB3C5]</p><p>
-  [Pcons+RBS+NcoI in PSB3C5]</p><p>
+  [P<sub>cons</sub>+RBS+NcoI in PSB3C5]</p><p>
-  [Pcons+RBS+NheI in PSB3C5]</p><p>
+  [P<sub>cons</sub>+RBS+NheI in PSB3C5]</p><p>
   nine kinds of PBAN</p><p>
 <font color="yellow">DNA Sequencing of</font></p><p>
-   [Pcons+RBS+NcoI-5+J61048]</p><p>
+   [P<sub>cons</sub>+RBS+NcoI-5+J61048]</p><p>
 <font color="#FF0000"><span style="font-size:1cm;">10/8</span></font><br> </p><p>
 <font color="yellow">DNA Sequencing of</font></p><p>
 [PBAN NcoI]</p><p>
-[Pcons+RBS+SalI -1]</p><p>
+[P<sub>cons</sub>+RBS+SalI -1]</p><p>
-[Pcons+RBS+SalI-11]</p><p>
+[P<sub>cons</sub>+RBS+SalI-11]</p><p>
-[Pcons+RBS+NheI-6]</p><p>
+[P<sub>cons</sub>+RBS+NheI-6]</p><p>
-[Pcons+RBS+xhol-2+RBS+BFP+TER]</p><p>
+[P<sub>cons</sub>+RBS+xhol-2+RBS+BFP+TER]</p><p>
-[Pcons+RBS+SacI-11+RBS+BFP+TER]</p><p>
+[P<sub>cons</sub>+RBS+SacI-11+RBS+BFP+TER]</p><p>
-[Pcons+RBS+ Nhel-20+RBS+BFP+TER]</p>
+[P<sub>cons</sub>+RBS+ Nhel-20+RBS+BFP+TER]</p>
 <p>
 <font color="yellow">Ligation of</font></p><p>
-Pcons+RBS+NcoI on psb3c5</p><p>
+P<sub>cons</sub>+RBS+NcoI on psb3c5</p><p>
-Pcons+RBS+NcoI on psb3c5</p><p>
+P<sub>cons</sub>+RBS+NcoI on psb3c5</p><p>
 <font color="#FF0000"><span style="font-size:1cm;">10/9</span></font><br></p><p>
 ALL Nhel-9(10/7)x2</p><p>
@@ Line 226: / Line 226: @@
 <font color="yellow">Electrophoresis of</font></p><p>
 Gel 1</p><p>
-Well1	Marker</p><p>
+Well1 Marker</p><p>
-Well2	Pcons+RBS+NheI-20+RBS+BFP+TER</p><p>
+Well2 P<sub>cons</sub>+RBS+NheI-20+RBS+BFP+TER</p><p>
-Well3	Pcons+RBS+Hind-1+RBS+BFP+TER</p><p>
+Well3 P<sub>cons</sub>+RBS+Hind-1+RBS+BFP+TER</p><p>
-Well4	Pcons+RBS+KpnI-1+RBS+BFP+TER </p><p>
+Well4 P<sub>cons</sub>+RBS+KpnI-1+RBS+BFP+TER </p><p>
-Well5	Pcons+RBS+Ndel-1+RBS+BFP+TER</p><p>2
+Well5 P<sub>cons</sub>+RBS+Ndel-1+RBS+BFP+TER</p><p>2
-Well6	Pcons+RBS+NcoI-5+RBS+BFP+TER</p><p>
+Well6 P<sub>cons</sub>+RBS+NcoI-5+RBS+BFP+TER</p><p>
-Well7	Pcons+RBS+AseI ALL</p><p>
+Well7 P<sub>cons</sub>+RBS+AseI ALL</p><p>
-Well8	PBAN NcoI-12</p><p>
+Well8 PBAN NcoI-12</p><p>
-Well9	Pcons+RBS+NheI-6</p><p>
+Well9 P<sub>cons</sub>+RBS+NheI-6</p><p>
-Well10	Pcons+RBS+Hind-1</p><p>
+Well10 P<sub>cons</sub>+RBS+Hind-1</p><p>
-Well11	Pcons+RBS+NcoI-20</p><p>
+Well11 P<sub>cons</sub>+RBS+NcoI-20</p><p>
-Well12	Pcons+RBS+NcoI-2-2</p><p>
+Well12 P<sub>cons</sub>+RBS+NcoI-2-2</p><p>
-Well13	Pcons+RBS+Ndel-2-1</p><p>
+Well13 P<sub>cons</sub>+RBS+Ndel-2-1</p><p>
-Well14	Pcons+RBS+Nedl-2-2</p><p>
+Well14 P<sub>cons</sub>+RBS+Nedl-2-2</p><p>
-Well15	Pcons+RBS+SacI-1</p><p>
+Well15 P<sub>cons</sub>+RBS+SacI-1</p><p>
-Well16	Pcons+RBS+SacI-11</p><p>
+Well16 P<sub>cons</sub>+RBS+SacI-11</p><p>
-Well17	Marker</p><p>
+Well17 Marker</p><p>
 Gel 2</p><p>
-Well1	marker</p><p>
+Well1 marker</p><p>
-Well2	Pcons+RBS+NheI-11</p><p>
+Well2 P<sub>cons</sub>+RBS+NheI-11</p><p>
-Well3	Pcons+RBS+Hind3</p><p>
+Well3 P<sub>cons</sub>+RBS+Hind3</p><p>
-Well4	Pcons+RBS+KpnI-7</p><p>
+Well4 P<sub>cons</sub>+RBS+KpnI-7</p><p>
-Well5	Pcons+RBS+SacI-16</p><p>
+Well5 P<sub>cons</sub>+RBS+SacI-16</p><p>
-Well6	Pcons+RBS+SalI-12</p><p>
+Well6 P<sub>cons</sub>+RBS+SalI-12</p><p>
-Well7	Pcons+RBS+Xhol</p><p>
+Well7 P<sub>cons</sub>+RBS+Xhol</p><p>
 <font color="yellow">Transformation of</font></p><p>
-Pcons+RBS+SacI-3 on psb3c5</p><p>
+P<sub>cons</sub>+RBS+SacI-3 on psb3c5</p><p>
-Pcons+RBS+KpnI-1 on psb3c5</p><p>
+P<sub>cons</sub>+RBS+KpnI-1 on psb3c5</p><p>
-Pcons+RBS+NcoI-1 on psb3c5
+P<sub>cons</sub>+RBS+NcoI-1 on psb3c5
 </p><p>
@@ Line 263: / Line 263: @@
 <font color="#FF0000"><span style="font-size:1cm;">10/10</span></font><br></p><p>
 <font color="yellow">Transformation of </font></p><p>
-Pcons+RBS+Ndel on psb3c5</p><p>
+P<sub>cons</sub>+RBS+Ndel on psb3c5</p><p>
-Pcons+RBS+NcoI on psb3c5</p><p>
+P<sub>cons</sub>+RBS+NcoI on psb3c5</p><p>
 <font color="yellow">Electrophoresis of </font></p><p>
-Well1	marker</p><p>
+Well1 marker</p><p>
-Well2	Pcons+RBS+SacI-3 on psb3c5</p><p>
+Well2 P<sub>cons</sub>+RBS+SacI-3 on psb3c5</p><p>
-Well3	Pcons+RBS+KpnI-1 on psb3c5</p><p>
+Well3 P<sub>cons</sub>+RBS+KpnI-1 on psb3c5</p><p>
-Well4	Pcons+RBS+NcoI-1 on psb3c5</p><p>
+Well4 P<sub>cons</sub>+RBS+NcoI-1 on psb3c5</p><p>
@@ Line 279: / Line 279: @@
 <font color="yellow">PCR of and Electrophoresis of</font></p><p>
-Well1	Pcons+RBS+NcoI+psb3c5</p><p>
+Well1 P<sub>cons</sub>+RBS+NcoI+psb3c5</p><p>
-Well2	Pcons+RBS+Ndel+psb3c5</p>
+Well2 P<sub>cons</sub>+RBS+Ndel+psb3c5</p>
 <br></div>
 <div class="single note">