Team:TCU Taiwan/M13Phage
From 2014.igem.org
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However, if this <em>E.coli </em>has been transferred with a pBluescript II SK(-), gp2 will recognize intergenic region and make a nick on this plasmid. And then gp5 dimers will package the single strand of pBluescript because they believe this is their “genome”! In this situation, host cell will release phages but these phages do not contains their genome, they will carry phagemid instead. So they will not be able to make next generation after infection, so we call them “phagemid-carrying phage”.</p></font></td> | However, if this <em>E.coli </em>has been transferred with a pBluescript II SK(-), gp2 will recognize intergenic region and make a nick on this plasmid. And then gp5 dimers will package the single strand of pBluescript because they believe this is their “genome”! In this situation, host cell will release phages but these phages do not contains their genome, they will carry phagemid instead. So they will not be able to make next generation after infection, so we call them “phagemid-carrying phage”.</p></font></td> | ||
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Revision as of 14:29, 17 October 2014
M13 Phage |
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M13 Phage Mechanism | |||||
In our project, the CRISPR system is been transported by phage. So how can phage recognized which DNA it should package and spread? That is accessed by phagemid and helper phage. |
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Take M13KO7 helper phage and phagemid pBluescript II SK(-) as example, as we use them in our experiment. M13KO7 helper phage has complete coat proteins and a complete genome just like normal M13 phage. But its f1 ori has been inserted by a p15A ori and a kanamycin resistance gene. While in pBluescript, its structure is like a normal plasmid but carries two replication origin: a high efficient PUC ori for itself, and an additional normal f1 ori. |
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