Team:TCU Taiwan/M13Phage
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Revision as of 14:28, 17 October 2014
M13 Phage |
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M13 Phage Mechanism | |||||
In our project, the CRISPR system is been transported by phage. So how can phage recognized which DNA it should package and spread? That is accessed by phagemid and helper phage. |
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Take M13KO7 helper phage and phagemid pBluescript II SK(-) as example, as we use them in our experiment. M13KO7 helper phage has complete coat proteins and a complete genome just like normal M13 phage. But its f1 ori has been inserted by a p15A ori and a kanamycin resistance gene. While in pBluescript, its structure is like a normal plasmid but carries two replication origin: a high efficient PUC ori for itself, and an additional normal f1 ori. |
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