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| | </style> | | </style> |
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| - | <!--navigationbar--> | + | |
| - | | + | |
| - | <div id="navigationblock">
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| - | <img id="navbarlogo" src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg"/>
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| - | <a href="https://2013.igem.org/Team:Peking">Home</a>
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| - | </ul>
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| - | </li>
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| - | <a href="">Team</a>
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| - | <li><a href="https://2013.igem.org/Team:Peking/Team/Members">Members</a></li>
| + | |
| - | <li><a href="https://2013.igem.org/Team:Peking/Team/Notebook">Notebook</a></li>
| + | |
| - | <li><a href="https://2013.igem.org/Team:Peking/Team/Attributions">Attributions</a></li>
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| - | </ul>
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| - | </li>
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| - | <li id="PKU_navbar_Project" class="Navbar_Item">
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| - | <a href="https://2013.igem.org/Team:Peking/Project">Project</a>
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| - | <ul id="Project_Sublist">
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| - | <div class="BackgroundofSublist"></div>
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| - | <li><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Adaptors</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Peking/Project/Devices">Devices</a></li>
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| - | </ul>
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| - | </li>
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| - | <li id="PKU_navbar_Model" class="Navbar_Item">
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| - | <a href="">Model</a>
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| - | <ul id="Model_Sublist">
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| - | <div class="BackgroundofSublist"></div>
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| - | <li><a href="https://2013.igem.org/Team:Peking/Model">Band-pass Filter</a></li>
| + | |
| - | <li><a href="https://2013.igem.org/Team:Peking/ModelforFinetuning">Biosensor Fine-tuning</a></li>
| + | |
| - | </ul>
| + | |
| - | </li>
| + | |
| - | <li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:90px">
| + | |
| - | <a href="">Data page</a>
| + | |
| - | <ul id="DataPage_Sublist">
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| - | <div class="BackgroundofSublist"></div>
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| - | <li><a href="https://2013.igem.org/Team:Peking/DataPage/Parts">Parts</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Peking/DataPage/JudgingCriteria">Judging Criteria</a></li>
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| - |
| + | |
| - | </ul>
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| - | </li>
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| - | <li id="PKU_navbar_Safety" class="Navbar_Item">
| + | |
| - | <a href="https://2013.igem.org/Team:Peking/Safety">Safety</a>
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| - | <ul id="Safety_Sublist">
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| - | </ul>
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| - | </li>
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| - | <li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:120px">
| + | |
| - | <a href="https://2013.igem.org/Team:Peking/HumanPractice">Human Practice</a>
| + | |
| - | <ul id="HumanPractice_Sublist">
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| - | <div class="BackgroundofSublist"></div>
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| - | <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/Questionnaire">Questionnaire Survey</a></li>
| + | |
| - | <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/FactoryVisit">Visit and Interview</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/ModeliGEM">Practical Analysis</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/iGEMWorkshop">Team Communication</a></li>
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| - | | + | |
| - | </ul>
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| - | </li>
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| - |
| + | |
| - | </ul>
| + | |
| - | <a href="https://2013.igem.org/Main_Page"><img id="iGEM_logo" src="https://static.igem.org/mediawiki/igem.org/4/48/Peking_igemlogo.jpg"/></a>
| + | |
| - | </div>
| + | |
| - | <!--end navigationbar-->
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| - | | + | |
| - | <!--MajorBody-->
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| - | <div id="MajorBody">
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| - | <div id="ProjectTitle">
| + | |
| - | <img id="TitleBackground" src="https://static.igem.org/mediawiki/igem.org/c/c5/Peking2013_home_title.jpg" />
| + | |
| - |
| + | |
| - | <h1 id="ProjectName">Aromatics Scouts<img id="LittleScout" src="https://static.igem.org/mediawiki/igem.org/8/8a/Peking2013_home_Telescope.png" /></h1>
| + | |
| - | <h1 id="ProjectSubname">A Comprehensive Biosensor Toolkit for Profiling Aromatic Compounds in the Environment</h1>
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| - |
| + | |
| - | <p id="PreLocation">
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| - | <B>★Presentation:</B> Room 34-101, Saturday 12:30 PM, Session 2
| + | |
| - |     <B>★Poster:</B> Sunday, #13 (Stata)
| + | |
| - | </p>
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| - | | + | |
| - | | + | |
| - | | + | |
| - | </div>
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| - | <img id="MajorBodyBackground" src="https://static.igem.org/mediawiki/igem.org/9/97/Peking2013_Home_background.jpg" />
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| - | <div id="AbstractBox">
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| - | <div id="AbstractBackground"></div>
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| - | <p id="AbstractContent">Monitoring aromatic compounds in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.
| + | |
| - | <br/><br/>
| + | |
| - | Transcriptional regulators that each detect a specific class of aromatics were first bioinformatically determined; and then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and the orthogonality/crosstalk between them proved that these biosensors are very capable at profiling aromatics present in water.
| + | |
| - | <br/><br/>
| + | |
| - | Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable compounds into detectable compounds, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.
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| - | <br/><br/>
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| - | We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.
| + | |
| - | </p>
| + | |
| - | </div>
| + | |
| - | <div id="AppendixBox1" class="SmallBoxes" >
| + | |
| - | <h1 id="Appendix1Title"><i>Mining aromatics-sensing Biobricks <br/>from the genomic database</i></h1>
| + | |
| - | <div id="Appendix1Cover" >
| + | |
| - | <img id="AppendixImg1" src="https://static.igem.org/mediawiki/igem.org/e/ec/Peking2013_homepage_appendix1.jpg" />
| + | |
| - | <h1 class="AppendixHeadLine">Biosensor Mining</h1>
| + | |
| - | <img id="Appendix1Icon" class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/1/13/Peking2013_home_appendix1icon.png" />
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| - | <p>The core component of our biosensor toolkit is the transcriptional regulators that sense aromatics. For the comprehensiveness of aromatics-sensing, a data-mining process was conducted to mine transcriptional regulators for each typical class of aromatics from the database Uniprot. </p>
| + | |
| - | <a href="https://2013.igem.org/Team:Peking/Project/SensorMining">LEARN MORE</a>
| + | |
| - | </div>
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| - | </div>
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| - | <div id="AppendixBox2" class="SmallBoxes">
| + | |
| - | <h1 id="Appendix2Title"><i>High-performance, profile-<br/>specific biosensors</i> </h1>
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| - | <div id="Appendix2Cover" >
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| - | <img id="AppendixImg2" src="https://static.igem.org/mediawiki/igem.org/1/17/Peking2013_home_appendix2.jpg" />
| + | |
| - | <h1 class="AppendixHeadLine">Biosensors</h1>
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| - | <img id="Appendix2Icon" class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/b/b2/Peking2013_home_appendix2icon.png" />
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| - | <p>A comprehensive set of biosensor circuits have been implemented using the aromatics-sensing transcriptional regulators. Each biosensor has a specific aromatics-sensing profile. Furthermore, the orthogonality of their sensing profiles was carefully assessed for practical applications.</p>
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| - | <a href="https://2013.igem.org/Team:Peking/Project/BioSensors">LEARN MORE</a>
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| - | </div>
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| - | </div>
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| - | <div id="AppendixBox3" class="SmallBoxes">
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| - | <h1 id="Appendix3Title"><i>Converting the undetectable into<br/>the detectable</i></h1>
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| - | <div id="Appendix3Cover" >
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| - | <img id="AppendixImg3" src="https://static.igem.org/mediawiki/igem.org/1/1b/Peking2013_home_appendix3.jpg" />
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| - | <h1 class="AppendixHeadLine">Adaptors</h1>
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| - | <img id="Appendix3Icon" class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/c/cd/Peking2013_home_appendix3icon.png" />
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| - | <p> To expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were taken from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits.</p>
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| - | <a href="https://2013.igem.org/Team:Peking/Project/Plugins">LEARN MORE</a>
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| - | </div>
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| - | </div>
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| - | <div id="AppendixBox4" class="SmallBoxes">
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| - | <h1 id="Appendix4Title"><i>Rapidly determining <br/> the analyte concentration</i></h1>
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| - | <div id="Appendix4Cover" >
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| - | <img id="AppendixImg4" src="https://static.igem.org/mediawiki/igem.org/e/e6/Peking2013_home_appendix4.jpg" />
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| - | <h1 class="AppendixHeadLine">Band-pass Filter</h1>
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| - | <img id="Appendix4Icon" class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/4/42/Peking2013_home_appendix4icon.png" />
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| - | <p>For the ease of practical analysis, a genetic device called "Band-pass Filter" has been constructed to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter can robustly quantify the aromatics in environmental samples. </p>
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| - | <a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">LEARN MORE</a>
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| - | </div>
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| - | </div>
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| - | <div id="AppendixBox5" class="SmallBoxes">
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| - | <h1 id="Appendix5Title"><i>Communications, Questionnaire survey, <br/>Factory Visit</i> and interview</h1>
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| - | <div id="Appendix5Cover" >
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| - | <img id="AppendixImg5" src="https://static.igem.org/mediawiki/igem.org/d/da/Peking2013_home_appendix5.jpg" />
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| - | <h1 class="AppendixHeadLine">Human Practice</h1>
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| - | <img id="Appendix5Icon" class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/8/85/Peking2013_home_appendix5icon.png" />
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| - | <p>To obtain the information about public awareness and the situation of aromatics pollution, a survey including factory visit and questionnaires has been conducted. We also guided an iGEM HS team and held "Model iGEM" as a competition without competitiveness.</p>
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| - | <a href="https://2013.igem.org/Team:Peking/HumanPractice">LEARN MORE</a>
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| - | </div>
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| - | </div>
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| - | <img id="AcknowledgementBox" src="https://static.igem.org/mediawiki/igem.org/c/c4/Peking_Sponsors.jpg">
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