Team:Wageningen UR/notebook/journal/resistance

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<dt id="02w3"><a>Growth experiments</a></dt>
<dt id="02w3"><a>Growth experiments</a></dt>
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<h3>High Performance Liquid Chromatography (HPLC)</h3>
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                                                        <p>Using a platereader, time based growth was measured on <i>P. putida</i>, to see the response to different concentrations of fusaric acid.
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<p>Since some soil bacteria are known to be able to degrade fusaric acid (FA), a HPLC experiment was started to test <i>P. putida</i>(PP) for fusaric acid degradation and carbon utilization. M9 media was used with glucose/fusaric acid(250ug/ml) or both as a carbon source.  </p>
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                                                        <img src="https://static.igem.org/mediawiki/2014/1/12/Wageningen_UR_journal_resistance_putida_growth.png" width="800px">
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                                                        <i>P. putida growth followed for 12 hours grown in LB medium. The OD was measured every 15 minutes at 600nm. Plates were incubated shaking at 30 degrees Celsius. Concentrations of fusaric acid are noted in the legend in ug/ml. </br>
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                                                        At concentrations higher then 500 <i>P. putida</i> ceases to grow.
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                                                        </figure>
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Revision as of 12:49, 17 October 2014

Wageningen UR iGEM 2014

 

 

 

Journal

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May

Growth experiments

June

HPLC
Growth experiments
P. putida growth followed for 12 hours grown in LB medium. The OD was measured every 15 minutes at 600nm. Plates were incubated shaking at 30 degrees Celsius. Concentrations of fusaric acid are noted in the legend in ug/ml.
At concentrations higher then 500 P. putida ceases to grow.



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