"
Team:Evry/Notebook/Transformation/23-07-2014
From 2014.igem.org
(Difference between revisions)
| Line 18: | Line 18: | ||
<br> | <br> | ||
| - | + | <div align="center"> | |
| + | <img src="https://static.igem.org/mediawiki/2014/f/fa/GelPCRori.jpg" alt="Figure" /><br> | ||
| + | <b><u>Figure:</u></b> Result of ORI amplification in <i>Pseudovibrio denitrificans</i> (Pd), <i>Pseudovribrio ascidiaceicola</i> (Pa) and <i>E.coli</i> DH5a.<br> | ||
| + | </div> | ||
| + | <br> | ||
| + | The negative control with <i>E.coli</i> DH5a confirms that there is no amplification. For <i>Pseudovibrio</i> strains, we obtain one band for <i>Pseudovibrio denitrificans</i> and two for <i>Pseudovibrio ascidiaceicola</i>. <br> | ||
| + | <br> | ||
| + | <br> | ||
Latest revision as of 12:44, 17 October 2014
Construction of a new plasmid - Choice and amplification of the ORI
PCR with
Migration of the PCR products, we have two bands per well.
Figure: Result of ORI amplification in Pseudovibrio denitrificans (Pd), Pseudovribrio ascidiaceicola (Pa) and E.coli DH5a.
The negative control with E.coli DH5a confirms that there is no amplification. For Pseudovibrio strains, we obtain one band for Pseudovibrio denitrificans and two for Pseudovibrio ascidiaceicola.
July 23
