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Team:Evry/Notebook/Transformation/22-07-2014
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| - | <u> <b> <big><FONT COLOR=#003333>Construction of a new plasmid - Choice of the ORI</font></big></b> </u> <br> | + | <u> <b> <big><FONT COLOR=#003333>Construction of a new plasmid - Choice and amplification of the ORI</font></big></b> </u> <br> |
We chose to amplify the genes RepA, RepB and RepC. We find these three genes with the genome browser on the genome of <i>Pseudovibrio FOBEG1</i>.<br> | We chose to amplify the genes RepA, RepB and RepC. We find these three genes with the genome browser on the genome of <i>Pseudovibrio FOBEG1</i>.<br> | ||
| - | PCR with primers 49 and 50 (see primers table in <a href="https://2014.igem.org/Team:Evry/Notebook/Protocols">Protocols</a>) | + | PCR with |
| + | <li>primers 49 and 50 (see primers table in <a href="https://2014.igem.org/Team:Evry/Notebook/Protocols">Protocols</a>) | ||
| + | <li>Q5 high fidelity enzyme | ||
| + | <li>TM=55°C. | ||
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Revision as of 12:38, 17 October 2014
Construction of a new plasmid - Choice and amplification of the ORI
We chose to amplify the genes RepA, RepB and RepC. We find these three genes with the genome browser on the genome of Pseudovibrio FOBEG1.
PCR with
Migration of the PCR products, we have no band the amplification didn't work.
July 18
