Team:Evry/Notebook/Transformation/18-07-2014

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Revision as of 12:27, 17 October 2014

Picture

Construction of a new plasmid
We chose to amplify the tkt promoter of the transkelotase gene. We find this strong constitutive promoter with the genome browser on the genome of Pseudovibrio FOBEG1.
PCR with primers 5 and 6 (see primers table in Protocols).

July 18

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