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Team:Aberdeen Scotland/Attributions
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| - | <h1>Our Supporters</h1> | + | <h1>Attributions; what the Aberdeen iGEM team members did</h1> |
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| + | <h3>Alpha Team – Ag43 Protein</h3> | ||
| + | <p>Team Members: Ana Maria Cujba and Martyna Sroka carried out the following work. | ||
| + | Alpha team took an Ag43 part produced by the 2012 Hokkaido University iGEM team, the goal at first appeared simple – insert a FLAG-tag-multiple-cloning-site (FLAG+MCS) into Ag43. However, two further problems had to be overcome first: 1. The part had six additional Pst1 sites (non-compliant with RFC-10) which did not appear in the registry sequence. These sites had to be removed. 2. Ag43 has beta hairpins, these are somewhat like Velcro and stick the cells together so that can form biofilms, for our assay these would lead to false-positives and two of them had to be removed. Only after solving those two problems, alpha team proceeded to insert FLAG+MCS; they also experimentally characterized all Ag43-related parts.</p> | ||
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| + | <h1>Our Supporters</h1> | ||
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<h3>We would like to extend our thanks to the organizations and companies that supported us and enabled our team to participate in this year's iGEM competition.</h3> | <h3>We would like to extend our thanks to the organizations and companies that supported us and enabled our team to participate in this year's iGEM competition.</h3> | ||
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Revision as of 11:35, 17 October 2014
Attributions; what the Aberdeen iGEM team members did
Alpha Team – Ag43 Protein
Team Members: Ana Maria Cujba and Martyna Sroka carried out the following work. Alpha team took an Ag43 part produced by the 2012 Hokkaido University iGEM team, the goal at first appeared simple – insert a FLAG-tag-multiple-cloning-site (FLAG+MCS) into Ag43. However, two further problems had to be overcome first: 1. The part had six additional Pst1 sites (non-compliant with RFC-10) which did not appear in the registry sequence. These sites had to be removed. 2. Ag43 has beta hairpins, these are somewhat like Velcro and stick the cells together so that can form biofilms, for our assay these would lead to false-positives and two of them had to be removed. Only after solving those two problems, alpha team proceeded to insert FLAG+MCS; they also experimentally characterized all Ag43-related parts.
