Team:Korea U Seoul/Project/jc

From 2014.igem.org

(Difference between revisions)
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                 <div class="s">
                 <div class="s">
<ol type="1">
<ol type="1">
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<li>Team registration</li>
+
<li>Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected</li>
-
<p>donwdonw</p>
+
<p>- We have validated several of our BioBricks and Devices through experiments, see Submitted Parts for more info.</p>
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<li>Complete Judging form</li>
+
<li>Document the characterization of this part in the “Main Page” section of that Part’s/Device’s Registry entry</li>
-
<li>Team Wiki</li>
+
<p>- All our characterization documentation has been uploaded to Parts Registry and much of it is available at our Characterization Results page.</p>
-
<li>Present a poster and a talk at the iGEM Jamboree</li>
+
<li>Submit this new part to the iGEM Parts Registry</li>
-
<li>The description of each project must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services</li>
+
<p>- We have submitted several new parts to the Parts Registry, see Submitted Parts for more info.</p>
-
<li>Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines)</li>
+
<li>iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted</li>
 +
<p>- Throughout this wiki, we hope to have made it clear what impact this project could have on the environment, the safety and the ethics of this project, and how we have taken this into consideration. </p>
</ol>
</ol>
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                 <div class="s">
                 <div class="s">
<ol type="1">
<ol type="1">
-
<li>Team registration</li>
+
<li>Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system</li>
-
<p>donwdonw</p>
+
<p>- We have helped the Paris-Saclay iGEM team and LiU iGEM team with survey and BIT-China team by giving answers for questionnaire, Imperial_iGEM team by giving a comment on reddit.</p>
-
<li>Complete Judging form</li>
+
<li>iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Describe an approach that your team used to address at least one of these questions. Evaluate your approach, including whether it allowed you to answer your question(s), how it influenced the team’s scientific project, and how it might be adapted for others to use (within and beyond iGEM). We encourage thoughtful and creative approaches, and those that draw on past Policy & Practice (formerly Human Practices) activities.</li>
 +
<p>- Throughout this wiki, we describe approaches that we used to address some questions.</p>
<li>Team Wiki</li>
<li>Team Wiki</li>
<li>Present a poster and a talk at the iGEM Jamboree</li>
<li>Present a poster and a talk at the iGEM Jamboree</li>

Revision as of 11:28, 17 October 2014

Bronze Requirements 6/6

  1. Team registration

    2. - Welcome to iGEM 2014 ! Your iGEM 2014 team application was accepted by iGEM Headquarters on 2014-04-01 12:21:32 and your team registration fee has been received.

  2. Complete Judging form
  3. Team Wiki

    4. - As you can see, we have made our fantastic wiki.

  4. Present a poster and a talk at the iGEM Jamboree

    5. - We are looking forward to planning for a visit to the Giant Jamboree in Boston to present our project for the other teams.

  5. The description of each project must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services

    6. - We, our team member do all things that is described in wiki

  6. Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines)

    7. - BBa_K1377000, BBa_K1377001

Silver Requirements 4/4

  1. Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected

    2. - We have validated several of our BioBricks and Devices through experiments, see Submitted Parts for more info.

  2. Document the characterization of this part in the “Main Page” section of that Part’s/Device’s Registry entry

    3. - All our characterization documentation has been uploaded to Parts Registry and much of it is available at our Characterization Results page.

  3. Submit this new part to the iGEM Parts Registry

    4. - We have submitted several new parts to the Parts Registry, see Submitted Parts for more info.

  4. iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted

    5. - Throughout this wiki, we hope to have made it clear what impact this project could have on the environment, the safety and the ethics of this project, and how we have taken this into consideration.

Gold Requirements

  1. Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system

    2. - We have helped the Paris-Saclay iGEM team and LiU iGEM team with survey and BIT-China team by giving answers for questionnaire, Imperial_iGEM team by giving a comment on reddit.

  2. iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Describe an approach that your team used to address at least one of these questions. Evaluate your approach, including whether it allowed you to answer your question(s), how it influenced the team’s scientific project, and how it might be adapted for others to use (within and beyond iGEM). We encourage thoughtful and creative approaches, and those that draw on past Policy & Practice (formerly Human Practices) activities.

    3. - Throughout this wiki, we describe approaches that we used to address some questions.

  3. Team Wiki
  4. Present a poster and a talk at the iGEM Jamboree
  5. The description of each project must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services
  6. Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines)
Safety equipment
  1. Everyone must use appropriate safety equipment (Lab gowns, gloves, and goggles,etc) required for the experiment.
  2. Gloves are necessary when handling toxic materials or if there is a wound on the hand.
  3. Goggles must be worn while performing experiments with hazardous materials or splashes of microorganisms
Labatory Facilities
  1. Our lab has sturdy door, which cuts off the lab from outside, and entrance of people other than the lab members is strictly regulated.
  2. Our lab is equipped with two sinks with which people can wash hands and lab equipment.
  3. Our lab is spacious enough and can be easily cleaned. The lab is cleaned regularly and is kept tidy.
  4. Bench tops are always kept tidy and clean. They are impervious to water and resistant to heat and chemicals involved in most experiments done in the lab.
  5. Lab furniture is appropriate for withstanding anticipated loads and uses.
  6. We are readily available with safety manual and guideline, which we can use in case of accidents.
Simple Questions
1. Would any of your project ideas raise safety issues in terms of:
  1. Researcher Safety
    2. EtBr: Ethidium bromide is used as a fluorescent tag for gel electrophoresis in our lab. EtBr is known to negatively affect important biological processes, such as DNA replication and transcription, because it intercalates and deforms double-stranded DNA. Though we wash our agarose gels with EtBr solution, we strictly make sure that the solution does not make contact with human body by wearing gloves when handling the solution. The gels are safely discarded according to the safety guideline provided by the university.
  2. Public Safety
    3. Genetically modified bacteria are strictly prevented from being released out of the lab.
  3. Environment Safety
    4. We strictly follow the safety guideline provided by the university to take appropriate means of safely discarding all experiment waste.
2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, did you document these issues in the Registry? how did you manage to handle the safety issue? How could other teams learn from your experience?
  1. Did you document these issues in the Registry?
    2. Yes
  2. How did you manage to handle the safety issue?
    3. Our project includes transforming modified Pilin A gene cluster from Corynebacterium diphtheriae into Corynebacterium glutamicum to express pili with the genetically modified C. glutamicum.
    Toxin-emitting strain of C. diphtheriae can cause diphtheria by adhering to the nasopharyngeal tract with their pili and emitting toxin, which causes symptoms, such as sore throat and fever. Our team is well aware of the pathogenicity of C. diphtheriae, but we have definitely made sure that our experiments are safe and do not cause any threats to biosafety. First of all, we are using only a part of the genomic DNA of C. diphtheriae, NOT the whole organism. C. glutamicum, into which we are transforming DNA parts of C. diphtheriae, is a completely non-pathogenic species and does NOT express virulent toxin that causes diphtheria symptoms. Also, we do not use C. diphtheriae pili as it is but instead substitute the original pilin protein into other proteins, such as GFP. Furthermore, we are by all means following the biosafety guideline provided by Korea University and KCDC about dealing with the C. diphtheriae genomic DNA parts.
3. Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project? If no, which specific biosafety rules or guidelines do you have to consider in your country?
Yes, our project is under the supervision of Korea University Safety & Security Team. The team has raised no safety issue regarding our project.
References
  1. Cupillard, L. et al., 2005. Impact of plasmid supercoiling on the efficacy of a rabies DNA vaccines to protect cats. Vaccine 23: 1910-1916.
  2. JM Tiedje et al., 1989. The planned introduction of genetically engineered organisms: Ecological considerations and recommendations Ecology, 70(2), 1989, pp. 298-315
  3. Efstathia V. Scoulica. et al., Spread of blaVIM-1-producing e. coli in a university hospital in Greece. Genetic analysis of the integron carrying the blaVIM-1 metallo-β-lactamase gene,Diagnostic Microbiology and Infectious Disease Volume 48, Issue 3, March 2004, Pages 167-172

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