"
Team:Evry/Notebook/CellCharacterization/Antibiotic test
From 2014.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
<html> | <html> | ||
| - | + | <div align="center"> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<h4 class=title4> <FONT COLOR=#0099CC><u> Preparation of antibiotic stocks </u> </font></h4> | <h4 class=title4> <FONT COLOR=#0099CC><u> Preparation of antibiotic stocks </u> </font></h4> | ||
| + | </div> | ||
| + | |||
<p> | <p> | ||
<table border="1"> | <table border="1"> | ||
Revision as of 10:37, 17 October 2014
Preparation of antibiotic stocks
| Antibiotic | Stock concentration | Protocol |
| Kanamycin | 25 mg/mL | Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water. Vortex 5min and filtration with a 200nm filter. Stock : -20°C (aliquots of 1mL) |
| Streptomycin | 100 mg/mL | Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water. Vortex 5min and filtration with a 200nm filter. Stock : -20°C (aliquots of 1mL) |
| Amplicilin | 100 mg/mL | Weight 1g of Amplicilin powder, solubilization into 20mL of miliQ water. Vortex 5min and filtration with a 200nm filter. Stock : -20°C (aliquots of 1mL) |
| Tetracyclin | 15 mg/mL | Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%. Vortex 5min and filtration with a 200nm filter. Stock : -20°C and hiden from light(aliquots of 1mL) |
| Chloramphenicol | 34 mg/mL | Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%. Vortex 5min and filtration with a 200nm filter. Stock : -20°C and hiden from light(aliquots of 1mL) |
