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Team:Pitt/Skin Probiotic/Melanin/Methods
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<p>Upon receiving the MelA part BBa_K193602 we transformed them into Mach1 cells, mini-prepped the plasmid and performed a diagnostic digest for the part. As can be seen in Figure # below no MelA part was observed indicating an issue with the iGEM stock of these parts. At this point we had to put this project on hold. </p> | <p>Upon receiving the MelA part BBa_K193602 we transformed them into Mach1 cells, mini-prepped the plasmid and performed a diagnostic digest for the part. As can be seen in Figure # below no MelA part was observed indicating an issue with the iGEM stock of these parts. At this point we had to put this project on hold. </p> | ||
| + | <center> | ||
| + | <img src = "https://static.igem.org/mediawiki/2014/0/05/Pitt_melanin_methods.jpg"> | ||
| + | <p><b>Figure 2. Gel of BBa_K193602 expected to contain melA with XbaI and PstI shows that the expected insert band (1896bp) is not present.</b></p> | ||
| + | </center> | ||
<hr> | <hr> | ||
Revision as of 10:33, 17 October 2014
Methods
Upon receiving the MelA part BBa_K193602 we transformed them into Mach1 cells, mini-prepped the plasmid and performed a diagnostic digest for the part. As can be seen in Figure # below no MelA part was observed indicating an issue with the iGEM stock of these parts. At this point we had to put this project on hold.
Figure 2. Gel of BBa_K193602 expected to contain melA with XbaI and PstI shows that the expected insert band (1896bp) is not present.
Timeline
Designing MelA system – 1 week
Receiving MelA parts from iGEM – 1 week
Characterizing MelA parts – 1 week
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