Team:Melbourne/Protocol
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Revision as of 10:31, 17 October 2014
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PROTOCOLS
PREPARING CELLS FOR TRANSFORMATION (A)
A1V1 Preparing Competent Cells
A2V1 Preparation of Liquid Broth Media
PREPARING PLASMIDS FOR TRANSFORMATION (B)
B6V1 RE-Based Colony Screening
TRANSFORMATION AND PROTEIN EXPRESSION (C)
PROTEIN PURIFICATION (D)
D1V1 Column Purification of His-Tagged Proteins
PROTEIN ANALYSIS (E)
OTHER (F)