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Team:Groningen/Template/MODULE/Notebook/bandage/week9
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| - | + | After several weeks of trying to pour cells inside the gel it's time to switch to another kind of experiments. | |
| + | This time we will start by doing nisin diffusion tests. We'd like to see if nisin diffuses through our acrylamid gel and still spreads out to other bacteria. | ||
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| - | + | how will we do this: We will start by pouring gm17 agar plates with the nz9000 strain on it, this is a strain that doesn't have any nisin resistance. While pouring we had 3 empty circles inside this agar plate where we can put in 3 small samples of acrylamid gel. | |
| + | This acrylamid gel contains the NZ9700 strain, this is a strain that can create nisin. It does this after induction with nisin. | ||
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| - | + | The acrylamid gels which we will put inside this agar plate have several variations. We can choose to induce them, but since it's a leaky promotor we will try it without inducing as well. One of the other varaiables is that we let is grow overnight in either water or media (GM17) and this will likely give a different result. | |
| + | Our first try didn't go to well because the gel didn't seem to touch the agar, diffusion wouldn't start. | ||
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Revision as of 10:20, 17 October 2014
1 September - 4 September
After several weeks of trying to pour cells inside the gel it's time to switch to another kind of experiments.
This time we will start by doing nisin diffusion tests. We'd like to see if nisin diffuses through our acrylamid gel and still spreads out to other bacteria.
how will we do this: We will start by pouring gm17 agar plates with the nz9000 strain on it, this is a strain that doesn't have any nisin resistance. While pouring we had 3 empty circles inside this agar plate where we can put in 3 small samples of acrylamid gel.
This acrylamid gel contains the NZ9700 strain, this is a strain that can create nisin. It does this after induction with nisin.
The acrylamid gels which we will put inside this agar plate have several variations. We can choose to induce them, but since it's a leaky promotor we will try it without inducing as well. One of the other varaiables is that we let is grow overnight in either water or media (GM17) and this will likely give a different result.
Our first try didn't go to well because the gel didn't seem to touch the agar, diffusion wouldn't start.
