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Team:Paris Bettencourt/Parts
From 2014.igem.org
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<div id=project1><h6>Smell the roses</h6> | <div id=project1><h6>Smell the roses</h6> | ||
| - | <div class=part id=part1><p><a href="http://parts.igem.org/Part:BBa_K1403003">BBa_K1403003</a> - Limonene synthase</ | + | <div class=part id=part1><p><a href="http://parts.igem.org/Part:BBa_K1403003"><u>BBa_K1403003</a> - Limonene synthase</u></p></br>This part consists of a limonene synthase coding sequence downstream of a constitutive promoter from the Anderson's promoter library. We added a promoter and a RBS site to the original part Bba_I742110, making it directly expressed in E.Coli. </br></br>The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library.</br></br> |
We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later. Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.</br></br> | We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later. Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.</br></br> | ||
Revision as of 10:06, 17 October 2014
| SMELL THE ROSES | SOMETHING FISHY DON'T SWEAT IT | INTERLAB STUDY |
Smell the roses
BBa_K1403003 - Limonene synthase
This part consists of a limonene synthase coding sequence downstream of a constitutive promoter from the Anderson's promoter library. We added a promoter and a RBS site to the original part Bba_I742110, making it directly expressed in E.Coli. The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library. We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later. Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent. The experiment process was completed by standard cloning. The promoter and RBS was designed as oligos and later aligned, digested and ligated. The finished plasmid is in standard biobricks format.Part 2
Part 3
Part 4
Part 5
Part 6
Something fishy
Part 7
Don't sweat it
Part 8
Interlab study
Part 9
Part 10
+33 1 44 41 25 22/25
