Team:Virtus-Parva Mexico/Notebook
From 2014.igem.org
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+ | <h1><i>Biology Notebook</i></h1></div> | ||
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+ | <p><div align="justify"><div style="margin-left: 100px;"> | ||
+ | <h5><b> 29/05/2014</b></h5></p> | ||
+ | <br> For the first day of wetlab, we prepared the solutions we would use throughout our project. | ||
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+ | <br> <ul><li>TE Buffer Recipe:</li> | ||
+ | <ol><li>10 mM Tris, pH 8 (with HCl)</li> | ||
+ | <li>1 mM EDTA</li></ol> | ||
+ | <li>To make the CTAB/NaCl solution:</li> | ||
+ | <ol><li>4.104 g of NaCl</li> | ||
+ | <li>Slowly add 10.004 g CTAB</li> | ||
+ | <li>Add 60 ml of water</li> | ||
+ | <li>Heat at 74 °C</li></ol></ul> | ||
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+ | <br> After, we prepared a solution of EDTA 0.5 M by adding 3.65 grams in 25 mL of water. | ||
+ | <br> The Tris 1M solution took 3.02 grams in 25 mL water. These would be further diluted later on. | ||
+ | <br> A mistake was made within the calculations, a redo would have to be performed. The correct amount of EDTA and Tris were weighed and left for later. | ||
+ | <br> The day lasted longer than anticipated, the task list involved: | ||
+ | <ul type="square"><li>Hydration of the EDTA and Tris</li> | ||
+ | <li>Review Tris’ pH with potentiometer</li> | ||
+ | <li>Mix TE buffer</li> | ||
+ | <li>Sterilize solutions</li></ul> | ||
+ | </td> | ||
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+ | <p><div align="center"><div style="margin-left: 100px;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/thumb/f/fe/Team_Virtus_Parva_Inorganica_Joy.jpg/600px-Team_Virtus_Parva_Inorganica_Joy.jpg" width="350" height="350" alt=""/> | ||
+ | </div></div> | ||
+ | </td></tr> | ||
Revision as of 09:47, 17 October 2014
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Notebooks
Inorganic Notebook
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03/07/2014
04/07/2014
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07/07/2014
SiO2, n = 1.46 2.42 + 1.46 = 3.15 08/07/2014
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09/07/2014
10/07/2014
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11/07/2014The samples were once again washed with water, these samples easily precipitated in water, contrary to previous samples. Code names for these samples were NB2_(1-3) To help the precipitation, a little amount of acetone was added, this was also done for sample SB2’s aliquots. The samples were magnetically decanted and dried under vacuum for 4 hours. |
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15/07/14Samples were taken of pure magnetite, silanized magnetite (NB2) and magnetite coated with silica and silane (SB2_1 and SB2_3) for its characterization in IR for solids. Comparing the spectra given by the IR of the pure magnetite and silanized magnetite (SB2 and NB2) we were able to distinguish a peak at 990.2 cm-1 corresponding to a Si-O bond, confirming the correct silanization of the magnetite, although the amino group couldn’t be identified. Similar results were achieved for samples SB2_1 and SB2_3, however, this peak was not decisive to know if the sample was correctly functionalized giving the fact that the samples are coated with silica. |
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Biology Notebook
29/05/2014For the first day of wetlab, we prepared the solutions we would use throughout our project.
After, we prepared a solution of EDTA 0.5 M by adding 3.65 grams in 25 mL of water. The Tris 1M solution took 3.02 grams in 25 mL water. These would be further diluted later on. A mistake was made within the calculations, a redo would have to be performed. The correct amount of EDTA and Tris were weighed and left for later. The day lasted longer than anticipated, the task list involved:
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