"
Team:Paris Saclay/Notebook/June/30
From 2014.igem.org
(Difference between revisions)
(→Monday 30th June) |
(→Monday 30th June) |
||
| Line 11: | Line 11: | ||
1mL of an overnight culture in 100mL of LB. | 1mL of an overnight culture in 100mL of LB. | ||
| - | |||
====2 - Réhydration of selected biobricks:==== | ====2 - Réhydration of selected biobricks:==== | ||
| Line 27: | Line 26: | ||
* BBQ_J45014 | * BBQ_J45014 | ||
* BBQ_K517003 | * BBQ_K517003 | ||
| - | |||
====3 - Transformations :==== | ====3 - Transformations :==== | ||
| Line 35: | Line 33: | ||
We use the DH5α provided by our advisors for the aforementioned Biobricks. | We use the DH5α provided by our advisors for the aforementioned Biobricks. | ||
Spread on petri dishes with the concentration 10^-1 and 10^-2. | Spread on petri dishes with the concentration 10^-1 and 10^-2. | ||
| - | |||
====4 - Preparations :==== | ====4 - Preparations :==== | ||
| Line 51: | Line 48: | ||
Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes. | Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes. | ||
| - | |||
'''People there:''' | '''People there:''' | ||
Revision as of 15:18, 11 July 2014
Contents |
Monday 30th June
Lab work
1 - Culture or the supercompetents:
by Mathias
- MG1655
- MG1655Z1
1mL of an overnight culture in 100mL of LB.
2 - Réhydration of selected biobricks:
by Juliette
- BBQ_I9026
- BBQ_K592025
- BBQ_K786002
- BBQ_K786003
- BBQ_K778002
- BBQ_K592011 {2 times}
- BBQ_K592009
- BBQ_K1033910 {idem}
- BBQ_J45014
- BBQ_K517003
3 - Transformations :
by Maher & Mathias
We use the DH5α provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10^-1 and 10^-2.
4 - Preparations :
- Aliquots of antibiotics :
by Marie & Mathieu
Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. (Do the same for Kanamycin and Ampicillin)
- Petri dishes
by Romain
Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes.
People there:
- Instructors : Solenne.
- Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
