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Team:Pitt/Skin Probiotic/Cathelicidin/Results
From 2014.igem.org
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<td> | <td> | ||
<p><b>Hsp60 (no RBS) and Hsp60 (RBS) parts:</b></p><br><br> | <p><b>Hsp60 (no RBS) and Hsp60 (RBS) parts:</b></p><br><br> | ||
| - | Lanes: 1         2         3         4       5       6 | + | Lanes: 1         2         3         4       5       6<br> |
<img src = "https://static.igem.org/mediawiki/2014/6/6c/Pitt_cath_results4.jpg" style = "height:2.3in;width:auto"></td> | <img src = "https://static.igem.org/mediawiki/2014/6/6c/Pitt_cath_results4.jpg" style = "height:2.3in;width:auto"></td> | ||
<td> | <td> | ||
<p style = "border: 2px solid black;">Lanes 1 and 5 have the 1 kb + ladder. Lanes 2, 3, and 4 have the Hsp60 promoter without RBS (~350 bp). Lanes 6 and 7 have the Hsp60 promoter with RBS (~380 bp).</p></td> | <p style = "border: 2px solid black;">Lanes 1 and 5 have the 1 kb + ladder. Lanes 2, 3, and 4 have the Hsp60 promoter without RBS (~350 bp). Lanes 6 and 7 have the Hsp60 promoter with RBS (~380 bp).</p></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> | ||
| + | <p><b>Blue Promoter (FixK2) – mRFP1 – Cathelicidin part:</b></p><br><br> | ||
| + | Lanes: 1         2         3         4       5       6<br> | ||
| + | <img src = "https://static.igem.org/mediawiki/2014/4/43/Pitt_cath_results5.jpg" style = "height:2.3in;width:auto"></td> | ||
| + | <td> | ||
| + | <p style = "border: 2px solid black;">Lane 1 contains the 1 kb+ ladder. Lane 2 contains the Blue Promoter – mRFP1 – Cathelicidin part (~1100 bp).</p></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 09:06, 17 October 2014
Results
mRFP1 Part:
Lanes: 1 2 3 4 5 6 7 |
In Lane 1 of this gel is the 1 kb+ ladder. Lanes 3, 5, and 7 all contain the mRFP1 plasmid digested with XbaI and PstI. Each lane has two bands. The first at around ~2100 bp corresponds to the vector backbone. The second is a little above 700 bp, and corresponds to mRFP1 (770 bp). |
|
Blue Promoter (FixK2) Part: Lanes: 1 2 3 4 5  |
Lane 1 has the 1 kb+ ladder. Lanes 4 and 5 have the FixK2 part digested with XbaI and PstI. The band seen between 200 and 300 bp corresponds to FixK2 (250 bp). |
|
Blue Sensor (YF1 and FixJ) Part: Lanes: 1 2 3  |
In Lane 1 of this gel is the 1 kb+ ladder. Lanes 3, 5, and 7 all contain the mRFP1 plasmid digested with XbaI and PstI. Each lane has two bands. The first at around ~2100 bp corresponds to the vector backbone. The second is a little above 700 bp, and corresponds to mRFP1 (770 bp). |
|
Hsp60 (no RBS) and Hsp60 (RBS) parts: Lanes: 1 2 3 4 5 6  |
Lanes 1 and 5 have the 1 kb + ladder. Lanes 2, 3, and 4 have the Hsp60 promoter without RBS (~350 bp). Lanes 6 and 7 have the Hsp60 promoter with RBS (~380 bp). |
|
Blue Promoter (FixK2) – mRFP1 – Cathelicidin part: Lanes: 1 2 3 4 5 6  |
Lane 1 contains the 1 kb+ ladder. Lane 2 contains the Blue Promoter – mRFP1 – Cathelicidin part (~1100 bp). |
Conclusions
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