"
Team:Paris Saclay/Notebook/July/3
From 2014.igem.org
(Difference between revisions)
(→1 - Phages P1 transduction in MG1655 and MG1655Z1) |
(→Thursday 3rd July) |
||
| Line 4: | Line 4: | ||
===1 - Phages P1 transduction in MG1655 and MG1655Z1=== | ===1 - Phages P1 transduction in MG1655 and MG1655Z1=== | ||
| - | |||
| - | |||
In 2ml Eppendorf : | In 2ml Eppendorf : | ||
Revision as of 14:16, 11 July 2014
Thursday 3rd July
Lab Work
1 - Phages P1 transduction in MG1655 and MG1655Z1
In 2ml Eppendorf :
- 4 eppendorf MG1655 (0.1ml + 50µl CaCl)
- 4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)
Add 100µl of the different phages. Wait 20 minutes!
Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M Wait 1 or 2 hours at 37°C with agitation
Pour in LB Kan petri dishes, incubate at 37°C.(Turn the petri dishes over when there is no more liquid in!)
People there:
Instructors : Alice & Solenne. Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
