Team:Paris Saclay/Notebook/July/3

From 2014.igem.org

(Difference between revisions)
(Thursday 3rd July)
(Thursday 3rd July)
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'''People there:'''
'''People there:'''
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* Instructors : Solenne.
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:Instructors : Solenne.
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* Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
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:Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]

Revision as of 14:12, 11 July 2014

Thursday 3rd July

Lab Work

1 - Phages P1 transduction in MG1655 and MG1655Z1

Mathieu

In 2ml Eppendorf :

4 eppendorf MG1655 (0.1ml + 50µl CaCl)
4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)

Add 100µl of the different phages. Wait 20 minutes!

Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M Wait 1 or 2 hours at 37°C with agitation

Pour in LB Kan petri dishes, incubate at 37°C.(Turn the petri dishes over when there is no more liquid in!)


People there:

Instructors : Solenne.
Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.


Back to the calendar