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Team:Pitt/Skin Probiotic/Cathelicidin/Results
From 2014.igem.org
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| - | <td>Lanes: | + | <td> |
| + | <p><b>Hsp60 (no RBS) and Hsp60 (RBS) parts:</b></p><br><br> | ||
| + | Lanes: 1         2         3         4       5       6       7<br> | ||
<img src = "https://static.igem.org/mediawiki/2014/6/6c/Pitt_cath_results4.jpg" style = "height:2.3in;width:auto"></td> | <img src = "https://static.igem.org/mediawiki/2014/6/6c/Pitt_cath_results4.jpg" style = "height:2.3in;width:auto"></td> | ||
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Revision as of 08:51, 17 October 2014
Results
Cathelicidin Part:
mRFP1 Part:
Lanes: 1 2 3 4 5 6 7 |
In Lane 1 of this gel is the 1 kb+ ladder. Lanes 3, 5, and 7 all contain the mRFP1 plasmid digested with XbaI and PstI. Each lane has two bands. The first at around ~2100 bp corresponds to the vector backbone. The second is a little above 700 bp, and corresponds to mRFP1 (770 bp). |
Lanes: 1 2 3 4 5 |
Lane 1 has the 1 kb+ ladder. Lanes 4 and 5 have the FixK2 part digested with XbaI and PstI. The band seen between 200 and 300 bp corresponds to FixK2 (250 bp). |
|
Blue Sensor (YF1 and FixJ) Part: Lanes: 1 2 3  |
In Lane 1 of this gel is the 1 kb+ ladder. Lanes 3, 5, and 7 all contain the mRFP1 plasmid digested with XbaI and PstI. Each lane has two bands. The first at around ~2100 bp corresponds to the vector backbone. The second is a little above 700 bp, and corresponds to mRFP1 (770 bp). |
|
Hsp60 (no RBS) and Hsp60 (RBS) parts: Lanes: 1 2 3 4 5 6 7  |
Lanes 1 and 5 have the 1 kb + ladder. Lanes 2, 3, and 4 have the Hsp60 promoter without RBS (~350 bp). Lanes 6 and 7 have the Hsp60 promoter with RBS (~380 bp). |
Conclusions
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