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Team:Paris Saclay/Notebook/July/3
From 2014.igem.org
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(→1 - Phages P1 transduction in MG1655Z1) |
(→1 - Phages P1 transduction in MG1655Z1) |
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In 2ml Eppendorf : | In 2ml Eppendorf : | ||
| - | |||
**4 eppendorf MG1655 (0.1ml + 50µl CaCl) | **4 eppendorf MG1655 (0.1ml + 50µl CaCl) | ||
**4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl) | **4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl) | ||
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Add in each eppendorf : | Add in each eppendorf : | ||
| - | + | **1ml LB liquid | |
| - | ** 1ml LB liquid | + | **500µl citrate at 5.10^-3M |
| - | ** 500µl citrate at 5.10^-3M | + | |
'''Wait 1 or 2 hours at 37°C with agitation''' | '''Wait 1 or 2 hours at 37°C with agitation''' | ||
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | [https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | ||
Revision as of 13:44, 11 July 2014
Thursday 3rd July
Lab Work
1 - Phages P1 transduction in MG1655Z1
Mathieu
In 2ml Eppendorf :
- 4 eppendorf MG1655 (0.1ml + 50µl CaCl)
- 4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)
Add 100µl of the different phages. Wait 20 minutes!
Add in each eppendorf :
- 1ml LB liquid
- 500µl citrate at 5.10^-3M
Wait 1 or 2 hours at 37°C with agitation
