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Team:DTU-Denmark/Achievements/Medal fulfillings
From 2014.igem.org
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<li>As participants in the measurement track we have contributed to the interlab study, by transforming GFP expressed from different promoters into E. coli and measure fluorescence signal with BioLector and FACS. (link to interlab study results) </li> | <li>As participants in the measurement track we have contributed to the interlab study, by transforming GFP expressed from different promoters into E. coli and measure fluorescence signal with BioLector and FACS. (link to interlab study results) </li> | ||
</ul> | </ul> | ||
| + | <li>Document at least one new standard BioBrick Part central to your project </li> | ||
| + | <ul> | ||
| + | <li>We have submitted two BioBricks essential for our project to the iGEM Registry. This includes the Spinach2 in pSB1C3 backbone with and without a terminator (link to part registration). This BioBrick is new to the registry we have introduced two point mutations to remove an internal SpeI restriction site from the original sequence. The parts have been sequenced and demonstrated to be expressed and bind to the fluorophore DFHBI-1T in E. coli. the parts are registered as BBa_K1330000 and BBa_K1330001. </li> | ||
| + | <li>Furthermore we intended to streamline the Anderson promoter library by transforming promoters formerly present in plasmid J61002 into the iGEM standard backbone pSB1C3. This includes the promoters J23105, J23110, J23112 and J23116, now registered as K1330002, K1330003, K1330004 and K1330005 respectively. This will be a great advantage for future iGEM teams working with the Anderson promoter Library. </li> | ||
| + | </ul> | ||
</ol> | </ol> | ||
<br> | <br> | ||
Revision as of 08:27, 17 October 2014
- Home
- Overview
- Methods
- Achievements
- Team
At the Giant Jamboree, we received a gold medal and an Interlab Study Award. We also won the Overgrad Best Parts Collection.
Bronze medal requirements
The following 5 goals have been achieved or are expected to be fulfilled at the final Jamboree:- Team registration
- Complete Judging form.
- Team Wiki.
- Present a poster and a talk at the iGEM Jamboree.
- Participate in the Measurement Interlab Study
- As participants in the measurement track we have contributed to the interlab study, by transforming GFP expressed from different promoters into E. coli and measure fluorescence signal with BioLector and FACS. (link to interlab study results)
- Document at least one new standard BioBrick Part central to your project
- We have submitted two BioBricks essential for our project to the iGEM Registry. This includes the Spinach2 in pSB1C3 backbone with and without a terminator (link to part registration). This BioBrick is new to the registry we have introduced two point mutations to remove an internal SpeI restriction site from the original sequence. The parts have been sequenced and demonstrated to be expressed and bind to the fluorophore DFHBI-1T in E. coli. the parts are registered as BBa_K1330000 and BBa_K1330001.
- Furthermore we intended to streamline the Anderson promoter library by transforming promoters formerly present in plasmid J61002 into the iGEM standard backbone pSB1C3. This includes the promoters J23105, J23110, J23112 and J23116, now registered as K1330002, K1330003, K1330004 and K1330005 respectively. This will be a great advantage for future iGEM teams working with the Anderson promoter Library.
Silver medal requirements
Gold medal requirements
Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
+45 45 25 25 25
dtuigem14@gmail.com .
+45 45 25 25 25
dtuigem14@gmail.com . 
