Team:NU Kazakhstan/Modeling
From 2014.igem.org
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<p>We want to use the saliva because more than 400 types of proteins, including: Cancerous markers (CEA, SCC, IAP, Cyfra, p16, p53 and others - 81% sensitive), Diabetic markers (Procalcitonin, Carbonic anhydrase 6 and othres (more than 60)), Periodontal, disease markers (He lactoferrin, TIMP and others), Sjogren’s syndrome (decreased lipocalin 1, calgranulin and etc.), Markers for other systemic diseases, Different bacteria (Streptococcus mutans and lactobacilli) and viruses can be tested.</p> | <p>We want to use the saliva because more than 400 types of proteins, including: Cancerous markers (CEA, SCC, IAP, Cyfra, p16, p53 and others - 81% sensitive), Diabetic markers (Procalcitonin, Carbonic anhydrase 6 and othres (more than 60)), Periodontal, disease markers (He lactoferrin, TIMP and others), Sjogren’s syndrome (decreased lipocalin 1, calgranulin and etc.), Markers for other systemic diseases, Different bacteria (Streptococcus mutans and lactobacilli) and viruses can be tested.</p> | ||
<p><b>We expect</b> the secretion of proteins with dimerization capacity by the haemolysin type I transport system of Escherichia coli</p> | <p><b>We expect</b> the secretion of proteins with dimerization capacity by the haemolysin type I transport system of Escherichia coli</p> | ||
- | < | + | <ol><h5>Here is how we plan to prepare saliva for testing:</h5> |
<li>Centrifuge saliva samples (800 g, 10 min, 4°C), and suspend the pellets in 150 μl of lysis buffer (45 mM HEPES, 0.4 M KCl, 1 mM EDTA, 10% glycerol, pH 7.8). May add protease inhibitors if available (Halt Protease Inhibitor Cocktail, EDTA-Free (100X). Incubate for 30 min at room Temperature</li> | <li>Centrifuge saliva samples (800 g, 10 min, 4°C), and suspend the pellets in 150 μl of lysis buffer (45 mM HEPES, 0.4 M KCl, 1 mM EDTA, 10% glycerol, pH 7.8). May add protease inhibitors if available (Halt Protease Inhibitor Cocktail, EDTA-Free (100X). Incubate for 30 min at room Temperature</li> | ||
<li>Centrifuge the samples at (11 000 g, 10 min, 4°C)</li> | <li>Centrifuge the samples at (11 000 g, 10 min, 4°C)</li> |
Latest revision as of 08:24, 17 October 2014
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