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Team:Melbourne/Project
From 2014.igem.org
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<h1 >Project</h1> | <h1 >Project</h1> | ||
| + | <p><h2>PROTOCOLS | ||
| + | </h2> | ||
| + | <p><h3>PREPARING CELLS FOR TRANSFORMATION (A)</h3></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6c/MELBOURNE_A1V1_Preparing_Competent_Cells_Protocol.pdf">A1V1 Preparing Competent Cells</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6c/MELBOURNE_A1V1_Preparing_Competent_Cells_Protocol.pdfA2V1_Preparation_of_Liquid_Broth_Media_Protocol.pdf">A2V1 Preparation of Liquid Broth Media</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/b/b4/MELBOURNE_A3V2_Making_Agar_Plates_Protocol.pdf">A3V2 Making Agar Plates</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/f/f2/MELBOURNE_A4V1_Measuring_OD_Protocol.pdf">A4V1 Measuring OD</a></p> | ||
| + | <p> </p> | ||
| + | <p><h3>PREPARING PLASMIDS FOR TRANSFORMATION (B)</h3></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6f/MELBOURNE_B1V2_PCR_Protocol.pdf">B1V2 PCR Protocol</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/f/f5/MELBOURNE_B2V4_DNA_Gel_Electrophoresis_Protocol.pdf">B2V4 DNA Gel Electrophoresis</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/a/a8/MELBOURNE_B3V3_DNA_Gel_Purification_Protocol.pdf">B3V3 DNA Gel Purification</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/9/9e/MELBOURNE_B4V3_Restriction_Digestion_Protocol.pdf">B4V3 Restriction Digestion</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/2/24/MELBOURNE_B5V2_Ligation.pdf">B5V2 Ligation</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/3/34/MELBOURNE_B6V1_RE-Based_Colony_Screening_Protocol.pdf">B6V1 RE-Based Colony Screening</a></p> | ||
| + | <p> </p> | ||
| + | <p><h3>TRANSFORMATION AND PROTEIN EXPRESSION (C)</h3></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/a/a7/MELBOURNE_C1V2_Transformation_Protocol.pdf">C1V2 Transformation</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/9/98/MELBOURNE_C2V2_Protein_Expression_in_E._Coli.pdf">C2V2 Protein Expression</a></p> | ||
| + | <p> </p> | ||
| + | <p><h3>PROTEIN PURIFICATION (D)</h3></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/e/e1/MELBOURNE_D1V1_Column_Purification_of_His-Tagged_Proteins.pdf">D1V1 Column Purification of His-Tagged Proteins</a></p> | ||
| + | <p> </p> | ||
| + | <p><h3>PROTEIN ANALYSIS (E)</h3></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/1/1b/MELBOURNE_E1V2_SDS-PAGE_Protocol.pdf">E1V2 SDS-PAGE</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/7/79/MELBOURNE_E2V2_Coomassie_Staining_Protocol.pdf">E2V2 Coomassie Staining</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/c/cb/MELBOURNE_E3V6_Western_Blotting_Protocol.pdf">E3V6 Western Blotting</a></p> | ||
| + | <p> </p> | ||
| + | <p><h3>OTHER (F)</h3></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/a/a5/MELBOURNE_F1V1_pH_Meter_Protocol.pdf">F1V1 pH Meter</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/0/0a/MELBOURNE_F2V2_Cleanup_Protocol.pdf">F2V2 Cleaning Up</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/65/MELBOURNE_F3V1_Autoclave_Protocol.pdf">F3V1 Large Autoclave</a></p> | ||
| + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/1/19/MELBOURNE_F4V1_Autoclaving_Materials_-_small_autoclave.pdf">F4V1 Small Autoclave</a></p> | ||
| + | |||
| + | <p> </p> | ||
<h3> Project Description </h3> | <h3> Project Description </h3> | ||
Revision as of 07:33, 17 October 2014
| Home | Team | Official Team Profile | Project | Parts | Modeling | Notebook | Safety | Attributions | Public Outreach |  |
Project
PROTOCOLS
PREPARING CELLS FOR TRANSFORMATION (A)
A1V1 Preparing Competent Cells
A2V1 Preparation of Liquid Broth Media
PREPARING PLASMIDS FOR TRANSFORMATION (B)
B6V1 RE-Based Colony Screening
TRANSFORMATION AND PROTEIN EXPRESSION (C)
PROTEIN PURIFICATION (D)
D1V1 Column Purification of His-Tagged Proteins
PROTEIN ANALYSIS (E)
OTHER (F)
Project Description
Content
Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)
References
iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.
You can use these subtopics to further explain your project
- Overall project summary
- Project Details
- Materials and Methods
- The Experiments
- Results
- Data analysis
- Conclusions
It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.
It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.
