Team:UT-Dallas/Notebook/8-08

From 2014.igem.org

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<LI> Glycerol Stock of P+tcpS+RBS+eYFP
<LI> Glycerol Stock of P+tcpS+RBS+eYFP
<LI> Transformation of reporter vectors for ctxA, tcpJ, tcpP, tcpS
<LI> Transformation of reporter vectors for ctxA, tcpJ, tcpP, tcpS
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         <LI> Test digested gRNA vectors with 2% gel for i5.22.5,8,21,12,3,24.
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         <LI> Test digested gRNA vectors with 2% gel for i5.22.5,i5.22.8,i5.22.21,i5.22.12,i5.22.3,i5.22.24.
<LI> Test digested tcpS reporter vectors.  
<LI> Test digested tcpS reporter vectors.  
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<img class="pic" src="%http://i.imgur.com/61AwAAW.jpg" width="300px" align="right"/>
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       <img src="https://static.igem.org/mediawiki/2014/thumb/4/40/UTDiGEM_August8_Gel.JPG/800px-UTDiGEM_August8_Gel.JPG" style="border-width: 0px; display: inline; margin-left: 0px; margin-top: 0px;" height="400px">
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  <br> This is the nice 2% gel with semi-nice bands.
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  <br> This is the nice 2% gel with 50bp ladder.
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Latest revision as of 06:34, 17 October 2014

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Friday, August 08, 2014

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We have a set of reporter vectors that we grew and harvested the DNA last week. Today, to qualitatively check that we got what we want, we are going to test digest our reporter vectors. After the check, we will send our second batch of sequencing order.
Today's tasks:
  • Glycerol Stock of P+tcpS+RBS+eYFP
  • Transformation of reporter vectors for ctxA, tcpJ, tcpP, tcpS
  • Test digested gRNA vectors with 2% gel for i5.22.5,i5.22.8,i5.22.21,i5.22.12,i5.22.3,i5.22.24.
  • Test digested tcpS reporter vectors.


Our weird fish bone gel test. Look at the 2 log ladder!


This is the nice 2% gel with 50bp ladder.

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