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Team:Northwestern/CFPS
From 2014.igem.org
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<li>out of K12, DH5a, and BL21, BL21 expressed the most fluorescence</li> | <li>out of K12, DH5a, and BL21, BL21 expressed the most fluorescence</li> | ||
<li>the plasmid used was superfolder GFP from Jewett Lab</li> | <li>the plasmid used was superfolder GFP from Jewett Lab</li> | ||
| + | <li><b>conclusion</b>our lysates are function</li> | ||
</ul> | </ul> | ||
Revision as of 04:43, 17 October 2014
Cell-Free Protein Synthesis
20140723 Testing our lysates
- data from BL21 (E.Coli) lysates
- out of K12, DH5a, and BL21, BL21 expressed the most fluorescence
- the plasmid used was superfolder GFP from Jewett Lab
- conclusionour lysates are function
20140915 All of our lysates
- RBS B0034
- Tried all of our lysates
- green fluorescence indicates amount of transcription
- red fluorescence indicates amount of translation
- conclusion: the data is most likely statistically meaningless
